藻酸双酯钠对血管内皮细胞的生长及其与中性粒细胞黏附功能的影响

Effects of polysaccharide sulfate on the growth of vascular endothelial cell cultured in vitro and the adhesion of vascular endothelial cell to polymorphonuclear neutrophils

目的探讨藻酸双酯钠(polysaccharide sulfate,PSS)对体外培养血管内皮细胞生长以及对血管内皮细胞与中性粒细胞(polymorphonuclear neutrophils,PMN)黏附功能的影响。方法体外培养人类脐静脉血管内皮细胞(细胞株ECV-304),分别经50,100,150,200,250μg.mL-1不同终浓度PSS处理或不经PSS处理后,采用MTT法观察各组ECV-304生长情况。建立血管内皮细胞缺氧/复氧(Hypoxia/Reoxygenation,H/R)模型,检测经或不经H/R及PSS处理条件下培养的ECV-304与脑梗死病人外周血PMN的黏附率。结果PSS浓度≤200μg.mL-1各组ECV-304活力高于未经PSS处理的对照组,差异有统计学意义(P<0.01),其中PSS浓度为100μg.mL-1组最明显。PMN与非H/R处理的体外培养的ECV-304的黏附率在脑梗死组较健康组明显增高;ECV-304经H/R处理后与脑梗死病人PMN黏附率增高。体外培养的ECV-304无论是否经H/R处理,培养液中加入PSS(PSS终浓度100μg.mL-1)组与不加PSS组比较,ECV-304与脑梗死病人PMN的黏附率明显降低,差异有统计学意义(P<0.01)。结论适当浓度的PSS能促进体外培养正常血管内皮细胞的生长,降低H/R血管内皮细胞与中性粒细胞的黏附能力。

Objective To investigate the effects of polysaccharide sulfate（PSS）on the growth of vascular endothelial cell cultured in vitro and the adhesion of vascular endothelial cell to poly morphonuclear neutrophils （PMN）. Methods ECV-304, a kind of human umbilical vein endothelial cells, was cultured in vitro with different concentrations of PSS（0,50,100,150, 200,250g·mL^-1） , the growth of ECV-304 was observed by means of MTT. Hypoxia/ reoxygenation （H/R） model of ECV-304 was established, the adhesion rate of PMN from patients with cerebral infarction to cultured ECV 304 was detected with or without treatment of PSS and H/R. Results The growth of ECV-304 in PSS-treated subgroups （final concentrations： 50,100,150,200g·mL^-1） were much better than in non-PSS subgroup, especially in subgroup with PSS final concentration of 100g·mL^-1. The adhesion rate of cultured ECV- 304 to PMN from patients with cerebral infarction was higher significantly than that from the healthy subject, which was increased when ECV-304 being treated with H/R. The adhesion rate of cultured ECV-304（whether treated with H/R or not） to PMN was decreased significantly when ECV-304 being treated with PSS（100 g·mL^-1） . Conclusion The experiments in vitro showed that PSS could promote the growth of cultured ECV-304 and decrease the adhesion rate of H/R ECV-304 to PMN.