光照法研究超氧化物歧化酶及其模型化合物与超氧离子的反应动力学

A Study on Reaction Kinetics of Superoxide Dismutase and Its Model Compounds with Superoxide Ion by Illumination

用改进的光照法研究了超氧化物歧化酶及其模型化合物μ-桥基·二(2,6-二乙酰基吡啶)缩二丙二胺合铜(Ⅱ)(桥基为SCN^-,N_3^-,I^-,Br^-,Cl^-,OH^-)与超氧离子的反应动力学,测定了反应速率常数k_Q,结果表明,超氧化物歧化酶的k_Q与脉冲辐解法及黄嘌呤氧化酶法的结果一致。6种配合物中,Cu_2L(SCN)(ClO_4)_3的k_Q最大,Cu_2L(N_3)(ClO_4)_3的最小,并讨论了真k_Q不同的原因。

We studied the reaction kinetics of superoxide dismutase and its model compounds μ-bridge-bis-N,N' -1,3-propylene bis(2,6-diacetylpyridine)iminato copper( Ⅱ )(bridge being SCN-,N3-, I-, Br-,Cl-and OH-)by modified illumination method and the rate constants kQ of the reaction were obtained. kQ measured for superoxide dismutase is in agreement with those measured by pulse radiolysis and xanthine oxidase method. Among the six complexes , Cu2L(SCN)(ClO4) has a maximum value of kQ and Cu2L(N3)(ClO4)3 has a minimum one. The reason for different values of kQ was discussed based on the present results and previous paper.