双试剂自动分析法测定血清不饱和铁结合力:Nitro-PAPS直接光度法

A new automatic method for analysis of UIBC in serum with two reagents:Nitro-PAPS direct spectrophotometric method

目的:建立简便、灵敏的双试剂自动化分析法测定血清不饱和铁结合力(UIBC)。方法:在表面活性剂存在下,用2-(5-硝基-2-吡啶偶氮)-5-[N-正丙基-N-(3-磺酸丙基)氨基]苯酚(Nitro-PAPS)为显色剂测定血清UIBC。结果:该法线性范围0～95μmol/L,平均回收率为100.9%,批内变异系数(CV)和批间变异系数分别为0.019、0.036,与日本和光UIBC测定试剂盒比较具有良好的相关性,线性回归方程和相关系数分别为Y=1.0221X+0.2104,r=0.9950。102例健康人血清UIBC含量为32.1～51.1μmol/L(x±2s)。结论:用Nitro-PAPS双试剂自动化分析法测定血清UIBC,方法简便、灵敏可靠,适合临床应用。

Objective：To establish a simple, sensitive and automatic method of determination of UIBC in serum with two reagents. Methods：For determination of serum UIBC, Nitro -PAPS in the presence of surfactant was used. Results ：The method was liner in the range of 0 - 90 μmol/L, the average rate of recovery was 100.9% , the withinrun and between - run CV was 0. 019 and 0. 036,respectively. Comparing this method （Y） with Wako UIBC reagents （X）, we obtained the regression equation. Y= 1. 0221X ＋0. 2104, r =0. 9950. The reference interval （-↑x＋2s） was 32. 1 -51.1μmol/L （n = 102）. Conclusion：This method is simple, sensitive and suitable for clinical application.