血栓素前列环素在大鼠轻症急性胰腺炎向重症急性胰腺炎转变中的变化和意义

THE CHANGE AND SIGNIFICANCE OF TAX_2,PGI_2 IN THE CONVERSION OF MILD ACUTE PANCREATITIS TO SEVERE ACUTE PANCREATITIS IN RATS

目的本实验旨在探讨血栓素、前列环素在轻症急性胰腺炎(MAP)向重症急性胰腺炎(SAP)转变中的变化和意义。方法将SD大鼠40只按编号法随机分为假手术组(n=8)、MAP组(n=8)、SAP组(n=24)。经胆胰管逆行加压注入2.5%牛磺胆酸钠建立大鼠MAP模型,在此基础上通过暂时阻断脾下动脉引发缺血再灌注损伤微循环障碍以产生SAP模型。术后6h剖杀各组大鼠,检测各组大鼠血液中血栓素(TXA2)、前列环素(PG I2)的含量,并观察其病理改变。结果MAP组大鼠较假手术组胰腺病理组织学评分显著增高(P<0.05),血栓素与前列腺环素的比值(T/P)值则无明显差异;而SAP组(钳夹脾下动脉15、30、60 min)较MAP组T/P值、胰腺病理组织学评分均有显著增高(P<0.05)。结论胰腺微循环障碍作为持续和加剧损害因素贯穿于SAP发展的全过程,是MAP向SAP转化的重要因素。

Objective To investigate the change and significance of TXA2,PGI2 in the conversion of mild acute pancreatitis （MAP） to severe acute pancreatitis （ SAP）- Methods Forty SD rats were randomized into 3 groups ： shame-operation group （ n = 8）, mild acute pancreatitis group（ n = 8） and severe acute pancreatitis group （n = 24）. The animal model of MAP was induced by retrograde injection of 2. 5 % sodium taurocholate into biliopancreatic duct in rats. Pancreatic Ischemia-reperfusion microcirculatory disorder was caused by blocking the inferior splenic artery and removing the clamp after MAP induction. At 6h, groups of rats were sacrificed. TXA2/PGI2 leavels were measured in blood of each group rats, and histological changes of the pancreas were observed. Results Compared with rats in shame-operation group, pancreatic histopathological score was significantly higher in rats of MAP group （P 〈 0. 05）, and the value of TXA2/PGI2 did not change obviously （P 〉 0. 05 ）. But compared with rats in MAP group, pancreatic histopathological score and the value of TXA2/PGI2 were both higher in rats of SAP group （blocking the inferior splenic artery 15min, 30min.60min） （P 〈 0. 05）. Conclusion Pancreatic microcirculatory disorder plays a continuous and aggravative hurting role in the acting of SAP, it can induce the conversion of mild acute pancreatitis to severe acute pancreatitis.