CD117、C-erbB-2及Ki-67蛋白在原发性肝细胞性肝癌中的表达及意义

EXPRESSION OF CD117、C-ERB-2 AND KI-67 IN HEPATOCELLULAR CARCINOMA AND THEIR SIGNIFICANCE

目的探讨原发性肝细胞肝癌(HCC)中CD117、C-erbB-2及Ki-67蛋白的表达特点及其与临床病理的关系,为今后可能的分子靶向治疗提供实验依据。方法应用免疫组化Envion法,检测56例HCC中CD117及C-erB-2蛋白及Ki-67的表达,对照组为正常肝组织10例,肝硬化组织30例。结果CD117在HCC组、肝硬化组和正常肝组织表达阳性率分别为32.1%、33.3%和20%,CD117表达在三组间无显著性差异,但与HCC分期及组织学分级显著相关(P<0.05)。C-erB-2在HCC组、肝硬化组和正常肝组织表达阳性率分别为51.8%、63.3%和30%,三组之间存在显著性差异(P<0.05),C-erbB-2表达与组织学分级无关,但与临床分期密切相关。Ki-67在HCC组、肝硬化组和正常肝组织表达阳性率分别为65.8%、28.5%和19.3%,三组之间存在显著性差异(P<0.05)。结论CD117与C-erbB-2在HCC发生、发展过程中具有重要作用。

Objective To explore the expression of CD117,C-erbB-2 and Ki-67 in hepatocellular carcinoma （HCC） and its clinical significance. Methods The expression of CD117.C-erbB-2 and Ki-67 were evaluated by Envion immunohistochemical staining in 56 cases of HCC and 30 cases of liver cirrhotic specimens, as well as 10 cases of normal liver specimens, Results The positive expression of CDll7 was found in 32, 1% of HCC,33.3% of cirrhostic liver specimens, and 20% of normal liver specimens, respectively. There was no significant difference among the three groups, but the CD117 expression was significant associated with the histological grades and clinical stages. The positive expression of C-erbB-2 was 51.8%, 63.3% and 30% in HCC,cirrhotic liver specimens and normal liver tissues, respectively. Moreover,statistical difference was found in the expression of C-erbB-2 among three groups. The C-erbB-2 expression was not related to histological grades, but dramatically related to the clinical stages. The positive expression of Ki-67 was 65.8% ,28. 5% and 19. 3% in HCC,cirrhotic liver specimens and normal liver tissues,respectively, statistical difference was found in the expression of Ki-67 among three groups. Conclusion CD117 and C-erbB-2 may play important role in liver carcinogenesis and the progression of HCC ,and Ki-67 may be used as a prognostic index.