摘要
本试验对鸡病毒性关节炎病毒(AVAV)包头地区分离株(B-98株)进行总RNA的提取。参考GenBank中ARV-S1133株S1基因组序列设计两对引物,应用RT-PCR技术特异性地扩增病毒的S1基因的cDNA片段,将S1基因cDNA克隆到pGEM-TEasy载体后进行测序。ARV S1基因包含3个开放阅读框(ORF1,ORF2和ORF3),分别编码P10、P17和SigmaC蛋白。应用计算机软件把所测得的序列与参考毒株ARV-176,ARV-138,ARV-S1133,Muscovy duck reovirus strain YJL(MDRV-YJL)的S1基因3个阅读框的核苷酸序列进行比较分析。结果表明:AVAV B-98株与ARV-176株P10、P17和SigmaC蛋白的核苷酸序列的同源性最高,与ARV-S1133株、MDRV-YJL株的同源性次之,与ARV-138株的同源性最低。
In this study, the total RNA of Avian Viral Arthritis Virus （AVAV） of Chinese BaoTou isolates B-98 （B-98） was extracted. According to the S1 gene sequence of ARV-S1133 strain published by GeneBank, two pairs of primers were designed. Using RT-PCR method, the cDNA of S1 gene of this virus was retro-transcribed. From the cDNA, S1 gene was cloned into pGEM-TEasy plasmid and then sequenced. The S1 gene of ARV contains three open read frames （ORF1, ORF2 and ORF3）, respectively, encoding P10.P17 and Sigma C proteins. Using computer solftware, the acquired sequence of nucleotides was analyzed and compared with that of S1 genes of ARV-176, ARV-138, ARV-S1133, Muscovy duck reovirus strain YJL（MDRV-YJL）according to the order of the three ORFs. The results of comparative sequence analysis indicated that the sequence of nucleotide of the P10.P17 and Sigma C protein of AVAV B-98 had the highest homology with ARV-176 strain, the higher homology with ARV-S1 133 and MDRV-YJL and the lowest homology with ARV-138.
出处
《中国兽医杂志》
CAS
北大核心
2007年第6期6-8,共3页
Chinese Journal of Veterinary Medicine
基金
内蒙古自治区自然科学基金项目(200308020405)
