人肺癌染色体异倍性的初步研究

The study on the chromosome aneuploidy in human lung cancer

目的探讨间期核荧光原位杂交(FISH)检测肺癌印片标本染色体异倍体的可行性和其临床应用前景。方法选取7、8、9和12号染色体特异着丝粒 DNA 探针,用 FISH 技术检测46例肺癌印片标本的染色体异倍体。结果 46例肺癌印片标本均检出染色体异倍体,主要特征为7、8、和12号染色休增多及9号染色体丢失。7、8、9和12号染色体异倍体总检出率分别为:67.4%(31/46)、60.9%(28/46)、28.3%(13/46)和56.5%(26/31)。结论间期核 FISH 可以检测出肺癌印片标本中染色体异倍体,这些改变有望应用于肺癌早期诊断和预后判断。

Objective To study the feasibility of interphase fluorescence in situ hybridization （FISH） in detection of chromosomal aneuploidy in touch preparations of lung carcinoma and its clinic significance. Methods We examined 46 touch preparations of lung carcinomas by FISH with DNA specific for chromosomes 7, 8, 9 and 12 to detect chromosomal aneuploidy. Results Aneuploidy, mainly featured in gains of chromosomes 7, 8 and 12 and loss of chromosome 9 were observed in all of the touch preparations of the 46 cases with lung cancer. The rates of chromosomal aneuploidy detected by FISH were 67.4% （31/ 46）, 60. 9% （28/46）, 28. 3% （13/46） and 56. 5% （ 26/31 ） respectively. Conclusion Interphase FISH is feasible in detecting aneuploidy in touch preparations of lung cancer. These alterations may be applied to early diagnosis and predicting prognosis of lung cancer.