摘要
根据GenBank上已发表的水貂肠炎细小病毒基因序列,在其编码VP2结构蛋白基因的高度保守区内,设计合成1对特异性引物,建立水貂肠炎细小病毒PCR诊断方法。经过敏感性及特异性检测,该引物能与MEV标准株和地方分离株核酸发生特异性结合,扩增出一段长度为570bp的DNA片段,而与CDV、ADV等病毒的PCR反应均呈阴性。设计的该对引物可用于MEV的临床检测。
Two primers were designed and synthesized according to the nucleocapasid gene sequence of mink enteritis virus from GenBank , and used to detect mink enteritis virus. Special products of 570bp size were amplified by these primers from MEV vaccine and suspected cases but not from CDV, CAV and ADV strains.The results indicated that the PCR assay of MEV was proved to be specific and sensitive. It is feasible method for rapid diagnosis of MEV.
出处
《特产研究》
2007年第2期1-3,共3页
Special Wild Economic Animal and Plant Research
基金
科技部科研院所社会公益研究专项(2005DIB4J048)
