女性NGU与单纯解脲脲原体感染的分群分型初步研究

Study of the Biovars and Serotypes of Ureaplasma urealyticum in NGU

目的探讨女性非淋菌性尿道炎或宫颈炎(NGU)与解脲脲原体(Uu)分群和分型的关系。方法采用液体培养,A7固体分离,液体纯化的方法获得Uu纯菌株,分别对114例女性单纯Uu感染的NGU和113例单纯Uu感染的女性携带者的Uu做PCR分群和生长抑制试验分型。结果NGU组1群56例(49.12%),2群58例(50.88%)。正常组1群51例(45.13%),2群62例(54.87%);NGU组Uu分型阳性率最高依次为2型、7型、6型,正常人组最高依次为7型、2型、14型。结论Uu感染所致NGU与Uu分群关系不大。

Objective To study the relationship between nongonococcal urethritis （NGU） and biovars and serotypes of Ureaplasma urealyticum （Uu）. Method Ureaplasma urealyticum isolates were isolated by liquid culture, ATsolid isolation and liquid pure culture methods. All Ureaplasma urealyticum isolates were collected from 114 cases of NGU infected with simple Uu and 113 cases of female carries infected with simple Uu. The biovars and serotypes of Uu were identified by PCR and metabolite inhibition test. Result In the study group, 56 isolates were Ureaplasma parvum （49.12%）, and 58 were Ureaplasma urealyticum （50.88%）. In the control group, 51 isolates were Ureaplasma parvum （45.13%）, and 62 were Ureaplasma urealyticum （54.87%）. In the study group serotype 2, serotype 7, and serotype 6 were frequently found, while in the control group serotype 7, serotype 2, and serotype 14 were more frequent. Conclusion The biovars of Uu have no relationship with NGU.