脓毒症大鼠心肌细胞凋亡和p53蛋白表达相关性的研究

Correlation between the Expression of the p53 Protein and Myocardial Apoptosis in Septic Rats

目的研究脓毒症大鼠心肌细胞凋亡的变化及其与p53蛋白表达的关系。方法以盲肠结扎穿刺法复制脓毒症大鼠模型,以电镜和凋亡原位末端标记法(TUNEL法)检测其心肌细胞凋亡变化,用免疫组化方法检测p53蛋白的表达。结果一定时间内脓毒症大鼠心肌细胞凋亡率均明显高于正常对照组和假手术对照组(P均<0.05),p53蛋白表达阳性数均较正常对照或假手术组明显升高(P均<0.05),其变化与TUNEL法检测凋亡的结果一致(P<0.05)。结论细胞凋亡可能是脓毒症时心肌损害的机制之一,其调控基因p53的改变或许可以作为脓毒症病情改变的标志,可利用它对脓毒症进行干预,以改善脓毒症的预后。

Objective To study the correlation between myocardial apoptosis and the expression of the p53 protein in septic rats. Method The sepsis model was constructed by cecal ligation and puncture. The myocardial apoptosis was detected by electron microscopy and TdT-mediated dUTP Nick End Labeling （TUNEL）. The p53 protein was detected by immunohistochemistry method. SPSS 10.0 statistical software was used to analyzed the data. Result Within certain period, the myocardial apoptosis rates and expression of p53 protein in septic rats were higher than that in normal and sham-operated control group （P〈0.05）. The trend was parallel with the change of the results of TUNEL. Conclusion Myocardial apoptosis may contribute to the myocardial injury in sepsis. The p53 level may be able to indicate myocardial injury. The p53 gene may be used as a target for treating and prognosis of sepsis.