卫矛属植物组织培养无菌苗的方法研究

Approaches to get germ-free Euonymus plantlets through tissue culture

以卫矛属3种植物为试验材料,研究了取材方法、取材时间和不同品种对组培污染率的影响;采用茎段、小茎尖和嫩梢培养对卫矛属植物进行消毒处理,以其获得无菌苗。结果表明,宽瓣扶芳藤在春季3-4月腋芽萌发时进行消毒处理效果最好;胶东卫矛的消毒最为容易;以嫩梢培养方式进行消毒处理效果最佳。

Euonymus L. plants were sterilized in stem culture, caulody culture and branch culture. The experimental result indicated that： it＇s the best way for Euonymus fortunei Kuanban to be sterilized when axillary shoots were sprouting during March and April in Spring; Euonymus kiautschovicus was the easiest one to be sterilized among Euonymus plants; it＇ s the best way to choose sprouts culture to be sterilized.