高效液相色谱法定量分析1-氨基-2-羟基萘-4-磺酸

Determination of 1-Amino-2-hydroxylnaphthene-4-sulfonic Acid by Reversed-phase High Performance Liquid Chromatography

本文建立反相高效液相色谱法定量分析1-氨基-2-羟基萘-4-磺酸的分析方法。采用ODS-C18色谱柱,以甲醇/水为流动相,配比为25∶75,检测波长228nm,线性范围20.00mg/L～100.00mg/L,r=0.9999,标准偏差0.16,回收率99.86%～100.09%。分析精度好,满足工业产品质量检测的要求。

A simple and reliable high proformance liquid chromatography method, which is suitable for monoting the quality of the organic intermediate compound . It has been established on a spherisorb C18 column which the mobile phase of methanol water（25：75 ）and UV detection at 254 nm.