烟碱、高温及其联合作用对大鼠肝 GST、Cp和脂质过氧化反应的影响

Effects of nicotine and(or) hyperthermia on GST,Cp and lipid peroxidation in hepatocyte of rats

为探讨烟碱、高温及其联合作用对肝损伤的机制和敏感的检测指标，观察了烟碱（６．５ｍｇｋｇ－１ｄ－１灌胃，连续８天）或（和）高温（４２℃，６０分钟）处理大鼠肝线粒体、微粒体丙二醛（ＭＤＡ）、超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）和血清谷胱甘肽Ｓ转移酶（ＧＳＴ）、铜蓝蛋白（Ｃｐ）、丙氨酸转氨酶（ＡＬＴ）及细胞超微结构变化。结果发现：烟碱、高温均可导致肝微粒体脂质过氧化、微粒体ＳＯＤ的活力被抑制，两者具有协同作用；提高胞浆Ｃｐ活力，联合作用呈抑制效应。烟碱可抑制线粒体ＧＳＨ－Ｐｘ和胞浆ＧＳＴ活力。血清ＧＳＴ升高可能是热应激肝细胞受损的敏感指标。

To research for the mechanism and sensitive detection index of liver injury induced by nicotine or(and) hyperthermia,the serum and cytosolic glutathione S transferase(GST),ceruloplasmin(Cp),alanine aminotransferase(ALT),mitochondria and microsomal superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH Px) were detected.The ultrastructural changes of hepatocyte of rats were observed following heat stress(42℃ for 1 h) and(or) oral nicotine (6.5 mg·kg· -1 ·d -1 , for 8 d).The results indicated that microsomal lipid peroxidation formation was induced by nicotine or hyperthermia,SOD activity was inhibited more severe by their synergistic effect;cytosolic Cp activity was increased by each of them,but was inhibited by their combined effect.Mitochodrial GSH Px and cytosolic GST were inhibited by nicotine.Serum GST may be a sensitive index for detection of liver injury induced by heat stress.