摘要
采用两步法制备基因载体聚乙烯亚胺(PEI)改性纳米四氧化三铁(Fe3O4)磁性液体.样品经傅里叶红外光谱(FTIR)、Zeta电位分析、透射电镜(TEM)、热失重分析(TG)和磁化曲线测定等表征.改性水基磁性液体性能稳定,饱和磁化强度可达3.3emu/g.Fe3O4晶粒平均粒径大约为8nm,改性Fe3O4纳米颗粒粒径约为75nm,磁性液体的密度约为1.192g/cm3.用琼脂糖凝胶电泳方法定性分析了PEI改性水基磁性液体与DNA结合的情况,结果说明这种磁性样品可用作生物医学中基因载体.
A magnetic fluid modified with polyelthylenemine （PEI） was prepared by two-step methods. The samples were characterized by laser light scattering, transmission electron microscope （TEM）, Fourier transform infrared spectroscopy （FTIR）, thermogravimetric-differential thermal analysis （TG-DTA）, Zeta potential and vibration magnetometer, respectively. The PEI-modified magnetic liquid is stable and the saturation magnetization is 3.3 emu/g. The average grain size of Fe3O4 is about 8 nm. The nanoparticle size of the PEI-medi- fled magnetic fluid is about 75nm, and the density is 1.192 g/cm^3 , respectively. The connection between magnetic fluid and DNA was tested by electrophoresis. The results indicate that the PEI-medified magnetic liquid is a promising candidate as DNA cartier of biomedicine.
出处
《纳米技术与精密工程》
EI
CAS
CSCD
2007年第2期125-128,共4页
Nanotechnology and Precision Engineering
基金
国家自然科学基金资助项目(30672297)
广东省科技计划项目(2004A10703001)
