贫铀对体外培养成骨细胞的毒性作用

Studies on the Toxic Effects of Depleted Uranium on Osteoblast in Vitro

[目的]研究贫铀(depleted uranium,DU)对体外培养大鼠成骨细胞(osteoblast,OB)的毒性损伤作用,为DU对骨损伤防治提供依据。[方法]分离并培养原代成骨细胞,分别给予不同浓度(0.00195～0.0312mg/ml)的DU溶液,噻唑蓝(MTT)法检测细胞增殖率以观察DU对OB增殖的影响;对硝基苯磷酸二钠盐(PNPP)法测定细胞裂解液中碱性磷酸酶(ALP)活性以观察DU对OB分化能力的影响;矿化结节形成能力和面积测定以观察DU对OB矿化能力的影响。[结果]DU处理组OB增殖率、ALP活性均呈不同程度降低,且随着DU染毒剂量的增加和时间的延长,DU对OB增殖率和ALP活性的抑制作用更加明显,其中0.0078、0.0156、0.0312mg/ml等染毒剂量组与正常对照组相比,差异有统计学意义(JP<0.01)。此外,DU可明显抑制OB矿化结节形成能力,0.0039、0.0078mg/ml剂量组与对照组相比,差异有统计学意义(P<0.01)。[结论]DU在体外可以抑制OB增殖、分化和矿化能力,对OB的骨形成能力有明显抑制作用。

[Objective ] To study the acute toxic effects of depleted uranium（ DU ）on rat osteohlast（ OB ）in vitro. [Methods ] Osteohlasts were isolated from rat calvaria and cultured in vitro, then treated with different concentration of DU （ 0.00195-0.0312 mg/ml ）respectively. Osteohlast prolifereation was assessed with MTT, alkaline phosphatase activity of osteohlasts was measured with PNPP, and the capacity of mineralization was investigated by counting the area of bone nodules. [ Results] Compared with control group, the proliferation and the alkaline phosphatase activity of osteohlast were inhibited in DU treatment groups, with significantly lower levels observed in 0.007 8 mg/ml, 0.015 6 mg/ml and 0.0312 mg/ml DU treatment groups （P 〈 0.01 ）. With regard to capability of miheration, the area of nodules in osteohlast was significantly lower in 0.003 9 mg/ml and 0.007 8 mg/ml DU treatment groups than control group（ P 〈 0.01 ）. [ Conclusion ] DU could significantly inhibit the bone formation by inhibiting the proliferation, differentiation and mineralization capability of osteohlast in vitro.