高效液相色谱法测定保健食品中大豆异黄酮含量

Determination of Soybean Isoflavones in Health Foods by HPLC

建立了高效液相色谱法(HPLC)测定保健食品中大豆异黄酮含量的方法,运用二极管阵列检测器(PDA)建立了4种大豆异黄酮D、G、De、Ge的光谱库,测定了D、G、Ge与De响应值之比.采用PDA检测,C18反相柱,以甲醇∶水∶醋酸(HAC)=55∶45∶1(V/V/V)为流动相测定了同一批保健食品美龄营养片中4种大豆异黄酮D、G、De、Ge的含量分别为2.24%、10.82%0、.085%、0.027%,变异系数小于5%,回收率为92.51%～99.30%.该方法快速,精密度及准确度在允许范围内,可作为保健食品中D,G,De,Ge的同时测定方法.

A HPLC method for determination of soybean isoflavones including D, G, De, Ge in health foods was reported, The 1-4th derivative spectra of D, G, De, Ge was eatablished by PDA. The sample was analyzed on a C18 reversed--phase column （5 μm, 4. 6×250 mm） with a isogradient mobile of methanol ： water ： HAC=55： 45 ： 1 （V/V/V） . ISO in a health Meiling nutritions tablets was tested. The contents of D, G, De, Ge were 2. 24% , 10. 82%, 0. 085%, 0. 0027% respectively, and variation coefficient was less than 5%, and the recovery was 92.51%;99.30%. This method is simple, sensitivity, reproducibility and can be used for the determination of 4 soybean isoflavones in health foods.