安吉复注射液对小鼠实验性免疫功能低下的增强作用

Enhanced effect of An Ji Fu injection solution on experimental immunosuppression in mice

目的：研究安吉复注射液对小鼠实验性免疫功能低下的影响。方法：以环磷酰胺（Ｃｙ８０ｍｇ·ｋｇ－１ＳＣ）造成免疫功能低下的动物模型，采用碳粒廓清法、单向免疫扩散法、二硝基氟苯诱导迟发型超敏反应法、３Ｈ－ＴｄＲ参入法以及间接免疫荧光法测定有关免疫指标。结果：安吉复注射液（０．０１、０．１ｍｇ·ｋｇ－１·ｄ－１×１０ｄ，ｉｍ）可使小鼠降低的Ｋ值、α值、血清ＩｇＧ、耳片肿胀度、ＣｏｎＡ诱导的Ｔ淋巴细胞增殖反应值等明显升高；同时可使小鼠外周血减少的Ｌ３Ｔ４＋细胞增多，从而致降低的Ｌ３Ｔ４＋／Ｌｙｔ－２＋细胞比值增高。结论：安吉复注射液对小鼠实验性免疫功能低下有明显的增强作用。

Aim\ The effects of An Ji Fu injection solution AJF on experimental immunosuppression were studied in mice. Method\ The immunosuppressed mice were induced by cyclophosphamide (Cy, 80 mg·kg -1 sc). The immunomodulatory effects of the drug were measured by cleaning carbon particle method, simple diffusion method, delayed type hypersensitivity (DTH), 3H TdR entering method and indirect immunofluorescence assay. Result\ The k numble, α numble, the level of serum IgG and ear swelling were markely increased by An Ji Fu injection solution (0 01, 0 1 mg·kg -1 ·d -1 ×10 d, im). The drug could also enhanced the prolifevous reponse of T lymphocyte of peripheral blood induced by ConA. In addition, the drug could increase the number of L 3T + 4 cells of peripheral blood and the ratio of L 3T + 4/Ly 2 + cell. Concllusion\ AJF has a enhanced effect on experimental immunosuppression in mice.