染色体检查和AgNOR技术联合鉴别胸水细胞

Differential Diagnosis of Pleural Effusion by Combining Chromosome Analysis and AgNOR Technique

比较染色体检查和ＡｇＮＯＲ技术联合鉴别良恶性胸水诊断价值。方法常规染色体制片和Ｃｒｏｃｋｅｒ法，对４６例恶性胸水、７５例良性胸水患者进行染色体检查和ＡｇＮＯＲ技术联合鉴别。结果胸水细胞染色体检查敏感性９１．３％，准确率９３．４％；ＡｇＮＯＲ技术敏感性８９．１％，准确率９０．１％；恶性胸水和良性胸水ＡｇＮＯＲ每核平均数分别为５．８１±１．１９和１．８６±０．３８；染色体检查和ＡｇＮＯＲ技术联合鉴别敏感性９５．７％，准确率９８．３％。结论染色体异常和ＡｇＮＯＲ颗粒数增多，均与恶性肿瘤相关，二者联检比单纯染色体检查更有助于胸水性质的鉴别诊断。

Objective\ A comparative study on diagnosis of pleural effusion with chromosome analysis and AgNOR technique.\ Methods\ By using routine chromosome preparation and Crocker′s methods,chromosome analysis and AgNOR were examined in 121 pleural effusion cases(46 malignant cases,75 benign cases).\ Results\ Positivity rate and accuracy rate of chromosome analysis were 91.3% and 93.4% respectively,compared with 89.1% and 90.1% of AgNOR technique.\ The mean granules of AgNOR in malignant cases and in benign cases were 5.81±1.19 and 1.86±0.38 respectively. \ A total positivity rate of 95.7% and accuracy rate of 98.3% could be achieved when chromosome analysis and AgNOR technique were used in combination.\ Conclusion\ Chromosomal abnormalities and increasing granules of AgNOR were connected with malignant tumor.\ It is suggested that combining chromosome analysis with AgNOR technique can diagnose malignant pleural effusion more accurately.