IFNα A-HBV preS 融合蛋白在大肠杆菌中的表达及生物学活性的初步鉴定

The Expression and Biological Activity Identification of IFNα A HBV pre S Fusion Protein in E.Coli

本实验利用重组ＩＦＮαＡ－ＨＢＶｐｒｅＳ融合基因ｐｌｙ５质粒在大肠杆菌ＤＨ５α中表达ＩＦＮαＡ－ＨＢＶｐｒｅＳ融合蛋白。该融合基因产物的分子量为３９ｋＤ左右，融合蛋白的表达率为１１．８％。对该融合蛋白的初步鉴定表明：该融合蛋白既具有ＨＢＶｐｒｅＳ蛋白的免疫原性，又具备ＩＦＮα的生物学活性。

A recombinant fusion gene IFNα A HBV preS ply5 plasmid was introduced into E.coli DH5α strain to express the IFNαA HBV preS fusion protein. The molecular weight of the fusion protein was about 39 000 dalton. The rate of fusion protein expression was about 11.8% and the fusion protein was also been proved to have both IFNα biological activities and HBV preS immunogenicity.