氟喹诺酮类抗菌素-钯(Ⅱ)-曙红Y体系的共振瑞利散射光谱及其分析应用

Resonance Rayleigh Scattering Spectra of Some Fluoroquinolones Antibiotics-Pd (Ⅱ)-Eosin Y Systems and Their Analytical Application

在pH值为4.1-5.0的Britton-Robinson（BR）缓冲溶液中,环丙沙星（ciprofloxacin,CPF）、诺氟沙星（norfloxacin,NRF）、氧氟沙星（ofloxacin,OFL）、左氧氟沙星（levofloxacin,LVF）等氟喹诺酮类抗菌素（fluoro-quinolone derivatives,FQs）与Pd（Ⅱ）反应形成无色阳离子螯合物,当其与曙红Y反应形成三元离子缔合物,共振瑞利散射（RRS）均显著增强,并产生新的RRS光谱,最大RRS峰均位于368nm处。在一定范围内FQNs的浓度与RRS强度（ΔI）成正比,4种抗菌素的线性范围和检出限分别为0-2.4×10^-6g/mL和9.4×10^-9g/mL（CPF）;0-2.4×10^-6g/mL和12.8×10^-9g/mL（NRF）;0-2.2×10^-6g/mL和16.2×10^-9g/mL（LVF）;0-2.8×10^-6g/mL和15.6×10^-9g/mL（OFL）。并具有较好的选择性,用于针剂、鸡血清中诺氟沙星的测定时,其回收率在95.0%-101.5%。建立了一种灵敏、简便、快速测定喹诺酮类抗菌素的新方法。

In a Briton-Robinson（BR） buffer solution of pH 4.1 - 5.0, some iluoroquinolone derivatives（ norfloxacin, NRF; ciprofloxacin, CPF; ofloxacin, OFL; levofloxacin, LVF） were reacted with palladium（ H ） to form anion chelates, but their resonance Rayleigh scattering（RRS） was very weak. Significant enhancement of RRS intensity and new RRS spectra were observed when these anion chelates further reacted with eosin Y to form ternary complexes. These ternary complexes had similar spectral characteristics and their maximum RRS peaks were located at 368 nm. The optimum condition and the affecting factors were investigated. The linearity ranges and the detection limits for the four fluoroquinolone derivatives were 0 - 2. 4 ×10 -6 g/mL and 9. 4 × 10-9 g/mL（ CPF）, 0 ~2.4 × 10 -6 g/mL and 12. 8 ×10 ^-9 g/mL（ NRF）, 0 - 2. 2 × 10^ -6 g/mL and 16. 2 ×10^-9 g/mL（LVF） , 0 -2. 8 × 10 -6 g/mL and 15.6 ×10^-9 g/mL（OFL） , respectively. The composition of the ternary ion association complex was 1 ： 1 ： 1 and its reaction mechanism is discussed. The method has been successfully applied to the determination of NRF in injection and in chicken serum samples and its recovery ranges from 95.0% to 101.5%. As a result, a sensitive and selective RRS method has been developed for the determination of some fluoroquinolones antibiotics.