黄芩甙对柯萨奇Ⅲ型病毒性心肌炎病毒复制的影响

Study of Characteristics of Inhibition of Baicalin on CoxB3 Viruses in Vitro

目的采用观察细胞病理变化(CPE)的方法,探讨黄芩甙对柯萨奇Ⅲ型病毒复制的影响。方法通过细胞培养,用先吸附病毒再加入药物、先用药物处理后攻击病毒和直接灭活的方法,观察黄芩甙的药效学作用。结果在病毒先感染细胞后加入黄芩甙的实验中,低病毒感染量(10TCID50)时,显示出对柯萨奇Ⅲ型病毒有一定的抗病毒活性;在黄芩甙与病毒同时加入的实验中,在100TCID50、10TCID50时,均显示出对柯萨奇Ⅲ型病毒的抑制作用。结论黄芩甙对柯萨奇Ⅲ型病毒有一定的抗病毒活性。

Objective To study the characteristics of inhibition of baicalin on CoxB3 viruses in vitro. Method Methods of cells infected with viruses prior to drug adding or drug prior to viruses adding or virus and baicalin added on cells simultaneously were used to research pharmacedynamics effect of bacailin. Results Baicalin is used to treat african monkey kidney （Veto） cells with COX B3 virus infection simultaneously.The results showed that baicalin can inhibit cytopathic effect （CPE） of COX B3 virus when the virus infected prior to the drug, but it can inhibit CPE of COX B3 virus at 10TCID50, but not 100TCID50. Conclusion Baicalin has shown to inhibit the cytopathic effect of COX B3 viruses in vitro.