左心耳封堵术前后不同时期IL-6、CRP、VWF和Ps的变化

Changes of serum interleukin-6, creactive protein, von willebrand and P-selectin in pigs in different stages after left atrial appendage transcatheter occlusion

目的:探讨经胸猪左心耳封堵术后不同时期外周血白细胞介素-6(IL-6)、C反应蛋白(C-reactive protein,CRP)、von willebrang factor(VWF)、plasma P-selectin(Ps)的变化。方法:选用苏钟猪17只,一组12只采用开胸并经导管置入左心耳封堵器(封堵组),另一组5只仅开胸未行封堵(对照组),应用酶联免疫吸附试验(ELISA)测定血IL-6、CRP、VWF、Ps的变化。结果:封堵组IL-6、CRP、VWF、Ps水平在术后即刻较术前增加(P<0.05);术后1个月和3个月时的IL-6、CRP、VWF、Ps较术前比较无统计学意义(P>0.05)。对照组术后即刻IL-6、CRP、VWF、Ps水平较术前增加(P<0.05)。封堵组与对照组比较,术前和术后各时点IL-6、CRP、VWF、Ps水平无统计学意义(P>0.05)。结论:IL-6、CRP、VWF、Ps水平在左心耳封堵术后有一过性增高,其炎症反应与封堵无关,而与开胸手术有关,该封堵器引起的炎症反应及血管内皮损伤较轻,生物相容性好。

Objective：To study the changes and clinical significance of serum interleukin-6（IL-6）, C-reactive protein（CRP）,yon willebrang factor （VWF） and plasma P-selectin （Ps） in pigs in different stages after left atrial appendage transcatheter occlusion. Methods：Occlusion group with 12 shuzhong pigs and control group with 5 pigs were used in the experiment. The changes of serum IL-6, CRP, VWF and plasma Ps were measured by enzyme linked immunosorbent assay （ELISA） before the operation, immediately at the operation, and 1,3 month after the operation. Results：In occlusion group, the levels of serum IL-6, CRP, VWF and Ps were higher at immediate time after occlusion than those of before occlusion（P 〈 0.05）, but the levels of them at 1 month and 3 month after the occlusion were as high as those of before occlusion （P 〉 0.05）. In control group, the levels of serum IL-6,CRP,VWF and Ps were higher at immediate time after operation than those of before the left atrial appendage transcatheter occlusion （P 〈 0.05）. There were no significant difference in the levels of serum IL-6, CRP, VWF and Ps in different stages after the operation between the occlusion group and the control group （P 〉 0.05）. Conclusion：Levels of serum IL-6, CRP, VWF and Ps temporarily increased at immediate time after the operation. This observation showed the satisfactory biocompatibility, feasibility and safety of the domestic nitinol left atrial appendage occluder.