一种L-选择素配体合成酶在子宫内膜癌和正常子宫内膜中的差异表达

Different expression of a synthetic enzymen for L-selectin ligant in the endometrium from the patients with carcinoma of endometrium and normal women

目的:检测L-选择素配体合成的关键酶,N-乙酰氨基葡萄糖-6-磺基转移酶(N-acetyl-glucosamine-6-O-sulfotransferase),在子宫内膜癌和正常子宫内膜组织中的表达。方法:采用RT-PCR技术检测16例子宫内膜癌(研究组)和16例正常子宫内膜(对照组)组织中N-乙酰氨基葡萄糖-6-磺基转移酶的表达。结果:正常子宫内膜有N-乙酰氨基葡萄糖-6-磺基转移酶的表达;其表达的半定量值,癌组织为0.87±0.08,正常组织为0.45±0.11,癌旁组织为0.49±0.12,癌组织中明显高于正常子宫内膜,两组差异有显著性(P<0.001);癌组织中明显高于癌旁组织,两组差异有显著性(P<0.001)。结论:L-选择素配体合成酶—N-乙酰氨基葡萄糖-6-磺基转移酶,在子宫内膜癌中的高表达,可能与肿瘤的发生、发展和转移密切相关。

Objective：To investigate the expression of N-acetyl-glucosamine-6-O-sulfotransferase, an important enzyme in synthetic enzyme for L-selectin ligant in the endometrium from the patients with carcinoma of endometrium and normal women. Methods： Reverse transcription polymerase chain reaction （RT-PCR） method was used to detect and quantify N-acetyl-glucosamine-6-O- sulfotransferase mRNA expression levels in the endometrium of 16 patients with carcinoma of endometrium which were recruited as study group and 16 healthy women as the control. Results：The expression of N-acetyl-glucosamine-6-O-sulfotransferase was observed in normal, but the expression level in study group was significantly higher than that in control group（0.87 ± 0.08 vs 0.45 ± 0.11, P 〈 0.001）, higher than that in paraneoplastic endometrium of the patients with carcinoma（0.87 ± 0.08 vs 0.49 ± 0.12,P 〈 0.001）. Conclusion：The higher expression of N-acetyl-glucosamine-6-O-sulfotransferase in the endometrium from the patients with carcinoma of endometrium may indicate a close correlation with the development and metastasis of cancer cells.