摘要
本文利用透射电子显微镜对耳鲍(Haliotis asinina Linnaeus)精子的形态及超微结构进行了研究。研究结果表明:耳鲍的精子由头部、中段和尾部三部分组成,全长41.6μm。精子头部长1.8μm,头部由顶体、顶体下腔和细胞核组成,顶体电子密度比较均匀,呈圆锥形,长0.6μm,基部宽度为0.65μm,占头部长的1/3;顶体下腔长0.03μm,宽为0.65μm,腔中含有中等电子密度物质;细胞核圆棒状,长1.17μm,核中部的宽度为1.0μm。精子中段较短,长0.51μm,宽1.2μm,主要由5个线粒体包围一对中心粒构成。尾部是一根鞭毛,从前到后逐渐变细,鞭毛是由细胞质膜包被的轴丝组成,轴丝为典型的“9+2”微管结构,即轴丝是由两个中心微管及均匀分布在中心微管周围的9对双联体微管组成。因此,耳鲍与其它鲍类精子的基本结构相似,形态结构的主要差异表现在三个方面:一是耳鲍精子的头部似圆锥形,长1.8μm,是目前已研究的鲍类中头部最短的种类;二是耳鲍精子顶体长比其基部宽要小,顶体电子密度比较均匀,顶体与核的电子密度差异不明显;三是耳鲍精子中段线粒体的数量为5个,没有发现6个线粒体现象的存在。
Spermatozoa of the ear abalone Haliotis asinina Linnaeus were examined using transmission electron microscopy. The results showed that the spermatozoa of H. asinina Linnaeus were structurally simple (classic ect-aqua sperm). The total average length of each spermatozoan was 41.6μm. From anterior to posterior, the sperm consisted of three parts: the head, midpiece and end piece. The head consisted of a conical acrosome (0.6 μm in length, 0.65 μm in width, with a prominent subacrosomal cavity), a barrel-shaped nucleus (1.17 μm in length, 1.0 μm in width, with a prominent frontier nuclear fossa and a posterior fossa) . The electron density of the acrosome was comparatively homogeneous. A short midpiece (0.51μm in length, 1.2 μm in width) consisted of a pair of orthogonally arranged centrioles surrounded by a five spherical mitochondria. The end piece was a 39.8μm long flagellum (with "9 + 2" microtubular arrangement pattern axoneme) [ Acta Zoologica Sinica 53 (3) : 552 - 556, 2007 ].
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2007年第3期552-556,共5页
ACTA ZOOLOGICA SINICA
基金
国家高技术发展计划(863)(No.2004AA603130)
国家自然科学基金项目(No.40366001)
海南省外国专家局项目(No.20031211)资助~~
关键词
耳鲍
精子
微超结构
Ear abalone, Haliotis asinine, Spermatozoa, Ultrastructure