膜联蛋白Ⅰ在多种癌组织中的表达

Expression of annexinⅠin different histological types of carcinomas

目的检测分析多种癌组织中膜联蛋白(annexin)Ⅰ的表达情况,探讨其表达变化规律与不同器官的相同和不同组织学类型癌的发生发展的相关性。方法构建的628例癌组织芯片中,食管鳞癌208例,其他14种器官的相同或不同组织学类型癌组织420例。以相应正常组织作对照。用免疫组化方法检测annexinⅠ的表达情况。结果annexinⅠ在多器官(食管、肺、喉、宫颈)的鳞癌表达下降,分化差者表达更低;在多种腺癌(胃和结直肠腺癌、胰腺导管腺癌、甲状腺乳头状癌和肾透明细胞癌)中表达上调,分化差者表达更高。结论annexinⅠ在多种癌组织中的表达有明显差异,并与组织学类型和分化程度明显相关。annexinⅠ在多种癌的发生中可能有重要作用。

Objective To investigate the expression of annexin Ⅰ in esophageal squamous cell carcinoma （SCC） and carcinomas of other histological types in order to analyze the correlation between the expression of annexin Ⅰ and carcinogenesis. Methods First, a set of tissue microarray was established, which consisted of SCC from the esophagus （208 cases） , lung, larynx, cervix, and external genital organs; adenocareinomas from the lung, stomach, colon and rectum, liver, pancreas, breast, thyroid and kidney with 30 cases in each group, meanwhile, the corresponding normal tissue was also obtained for control. Immunohistocbemistry was used to detect the expression of annexin I in different types of carcinomas and the corresponding normal controls from different organs. The correlation between the expression of annexin Ⅰ and the clinicopathological feature was analyzed and compared, which included age, gender, differentiation grade and lymph node metastasis. Results It was found that the expression of annexin I was decreased in esophageal SCC, when compared with normal esophageal squamous epithelia （ P 〈 0. 001 ） , the similarity was also found in SCC of the lung, larynx and cervix. However, though negative in normal epidermis, annexin Ⅰ expression was detected in some cases with SCC from external genital organs. Annexin Ⅰ was found to be overexpressed in adenocareinomas of the lung, stomach, colon and rectum, liver, pancreas, breast, thyroid and kidney, particularly very strong expression of annexin Ⅰ was seen in lung adenocarcinoma, uterine endometrioid adenocareinoma and ovarian serous adenocarcinoma. Interestingly, it was found to be positive in all thyroid papillary carcinomas, but negative in all normal thyroid glands. However, annexin I expression was found to be negative in all hepatocellular carcinoma and normal bepatocytes;and it was only detected in myoepithelium of normal breast tissue, but not in ductal luminal cells, and rarely in infiltrating ductal adenocarcinoma. In SCC, annexin Ⅰ expression was stronger in well differentiated ones than that in the poorly differentiated ones. However, contrasting with SCC, in the adenocarcinomas from different organs, annexin Ⅰexpression was much stronger in poorly differentiated ones than that in the well differentiate ones, especially in the adenocarcinomas from stomach, colon and rectum, pancreas, ovarian and kidney. Conclusion Annexin I expression is quite different among different types of carcinomas, and is correlated with histopathological type and differentiation grade. Further study is needed to investigate its role in the carcinogenesis.