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ERIC-PCR技术特点及其应用 被引量：18

Characteristic and Application of the Enterobacterial Repetitive Intergenic Consensus-PCR
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摘要 ERIC序列是近年来发现的存在于原核生物基因组中一类短的重复序列。该序列在染色体上的分布和拷贝数具有种间的特异性,根据序列中心高度保守的44 bp的ERIC核心序列设计反向引物,可扩增出反映细菌基因组结构特征的谱带。由于ERIC-PCR快速简便,图谱重复性好,能用于细菌分类鉴定、环境监测、污染治理以及人和动物肠道菌群结构研究等方面。 Entembacterial Repetitive Intergenic Consensus（ERIC）, a kind of short duplication sequences, is found to exist in the genome of pmkaryotic organism. Distribution and copy of the sequence in chrornosome is interspecific specificity.According to the highly conservative core sequence,44bp,reverse primer is designed. ERIC-PCR can generate unique fmgerprints to reflect the structural feature of bacterial strains, and can identification species. ERICPER technique can be used in the identification of bacterium, environmental monitoring, investigation intestinal flora of human beings and animals,which is rapid, simple and repetitive.
作者 李犹平 倪学勤
机构地区 四川农业大学动物科技学院
出处 《安徽农业科学》 CAS 北大核心 2007年第18期5358-5360,共3页 Journal of Anhui Agricultural Sciences
基金 四川农业大学青年基金
关键词 ERIC IRU ERIC-PCR 特点 应用 ERIC IRU ERIC-PCR Characteristic Application
分类号 Q503 [生物学—生物化学]
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参考文献31

  • 1SHARPLES G J,LLOYD R G.A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes[J].Nucleic Acids Research,1990,18(22):6503-6508.
  • 2HULTON C S J,HIGGINS C F,SHARP P M.ERIC sequences:a novel family of repatitive elemennts in the genomes of Escherichia coli,Salmonella typhimurium and other enterobacteria[J].Molecular Microbiologv,1991,5(4):825-834.
  • 3曹又方,赵立平.ERIC序列在不同细菌基因组中分布的分析[J].山西大学学报（自然科学版）,2002,25(4):354-357. 被引量：19
  • 4BACHELLIER S,CLEMENT J M,HOFNUNG M.Short palindromic repetitive DNA elements in enterobacteria:a survey[J].Research in Microbiology,1999,150:627-639.
  • 5MERION E,BECERRIL B,VALLE F,et al.Deletion of a repetitive extrgenic palindromic(REP)sequence downstream form the structural gene of Escherichia coli glutamate dehydrogenase affects the sability of its mRNA[J].Gene,1986,50(2/3):305-309.
  • 6GILSON E,CLEMENT J M,PERRIN D,et al.Palindromic units:a case of highly repetitive DNA sequence in bacteria[J].Trends in Genetics,1987(3):226-230.
  • 7GILSON E,PERRIN D,HOFNUNG M.DNA polymerase I and a protein complex bind specifically to E.coli palindromic unit highly repetitive DNA:implications for.bacterial chromosome organization[J].Nucleic Acids Research,1990,18(13):3941-3952.
  • 8FRANKLIN R B,TAYLOR D R.Characterization of microbial communities using randomly amplified polymorphic DNA (RAPD)[J].Journal of Microbiological Methods,1999,35(3):225-235.
  • 9WIKSTROM P,ANDERSSON A C,FORSMAN M.Biomonitoring complex microbial communities using random amplified polymorphic DNA and principal component analysis[J].FEMS Microbiology Ecology,1999,28(2):131-139.
  • 10杨朝国,安乙敏.病原微生物分子生物学分型方法的选择[J].国外医学（病毒学分册）,2000,7(2):48-51. 被引量：5

二级参考文献59

  • 1萨姆布鲁克J 费里奇EF.分子克隆实验指南（第2版）[M].北京:科学出版社,1993.474-489.
  • 2中华人民共和国国家标准食品卫生检验方法[s].微生物学部分(GB4789.30-94)[M].北京:中国标准出版社,1994.90—94.
  • 3Ojeniyi B. Polyagglulinable pseudomonas aeruginosa from cystic fibrosis patients: a survey. APMIS, 1994, 46(suppl) : 1 - 44.
  • 4Debast SB, Meis JFGM, Melchers WJ, et al. Use of interrepeat PCR fingerprinting to investigate an Acinetobacter baumannii out-break in an interisive care unit. Scan J Infect Dis, 1996, 28(2):577 - 581.
  • 5Chatelut M, Doumes JL, Chabanon G, et al. Epidemiological typing of stenotrophomonas(Xanthomonas) maltophilia by PCR. J Chin Microbiol,1995, 33(4) :912 - 914.
  • 6Versalovic J, Koeuth T, Jupski J R. Distribution of repetitive DNA sequences in Eubacteria and application of fingerprinting of bacterial genomes[J ]. Nucleic Acids Research, 1991, 19:6823 -6831.
  • 7Jersek B, Tchemera E, Rijpens N, et al. Repetitive element sequence-based PCR for species and strain discrimination in the genus Listeria[J]. Letters in Applied Microbiology, 1996, 23:55 -60.
  • 8SHARPLES G J,LLOYD R G.A Novel Repeated DNA Sequence Located in the Intergenic Regions of Bacterial Chromosome[J].Nucl Acids Res,1990,18:6503-6508.
  • 9HULTON C S,HIGGINS C F.ERIC Sequences:a Novel Family of Repetitive Elements in the Genomes of Escherichia Coli,Salmonella Typhimurium and other Enterobateria[J].Mol Microbiol,1991,5:825-834.
  • 10VERSALOVIC J,KOEUTH T.Distribution of Repetitive DNA Sequences in Eubacteria and Application to Fingerprinting of Bacterial Genomes[J].Nucl Acids Res,1991,19:6823-6831.

共引文献138

同被引文献191

引证文献18

二级引证文献70

安徽农业科学
2007年 第18期

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