金边瑞香的组培快繁技术研究

Studies on the Tissue Culture and Rapid Propagation of Daphne odora Var. Marginata

以金边瑞香的茎段为外植体,在MS基本培养基中添加不同浓度的6-BA和NAA,采用侧芽诱导法快速繁殖,研究了金边瑞香的组培快繁技术。结果表明,最佳启动培养基为MS＋6-BA 1.0 mg/L＋NAA 0.1-0.2 mg/L,最佳增殖培养基是MS＋6-BA 2.0 mg/L＋NAA0.2 mg/L,最佳生根培养基为1/2MS＋NAA 0.5 mg/L,生根率100%,且根系发达。经过该试验的驯化移栽,试管苗成活率达85%以上。

With the stern segments of Daphne odora var.marginata as explants, the tissue culture and rapid propagation techniques of Daphne odora var. rnarginata were studied with lateral bud revulsion and adding different concentration of 6-BA and NAA on the MS medium. The results showed that the best initiated medium in culture was MS ＋ 6-BA 1.0 n lg/L ＋ NAA 0.1 - 0.2 mg/L and the best proliferation medium was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.2 mg/L. The optimum shoots rooting medium was 1/2MS ＋ NAA 0.5 mg/L, with the rooting of 100% and developed roots. The survival rate of test tube plantlets was over 85% after the plantlet acclimation transplanting experiment.