用PCR方法检测基因缺失或杂合性丢失的初步探讨

A STUDY ON DETECTING THE GENE DELETION OR LOSS OF HETEROZYGOSITY BY POLYMERASE CHAIN REACTION

对ＰＣＲ方法用于检测基因的缺失或杂合性丢失进行了初步探讨。通过选择ＰＣＲ循环数、选择内对照及筛选标本等手段，采用ＰＣＲ－Ｓｏｕｔｈｅｒｎ技术检测ＧＳＨ克隆７在鼻咽癌中的缺失状态，成功地检测到了１例标本中存在的缺失。这为ＰＣＲ－Ｓｏｕｔｈｅｒｎ技术检测基因的缺失或杂合性丢失的应用提供了可借鉴的实验手段和经验。

The authors investigated the possibility of using PCR to dsetect the deletion of gene or loss of heterozygosity (LOH) of gene in biopies. With some aids, such as selection of PCR cycle number, choice of internal control and screening of samples, the authors detected the deletion of GSH Clone 7 in nasophryngeal carcinoma (NPC) by PCR Southern, and successfully found one case which partially deleted GSH Clone 7. This result was confirmed by image densitometry. Through this study, an available method to detect the deletion of gene by PCR is provided.