序贯共培养体系对小鼠体外受精胚胎发育效果的影响

Effect of Sequential Co-culture System on the Development and Quality of Murine Embryos Fertilization in Vitro

采用体外分离培养的兔输卵管上皮细胞和子宫内膜细胞构建了序贯共培养体系,并与体外受精得到的受精卵进行共培养。试验结果表明,HTF液受精率显著高于T6液(P<0.05),更适用于卵母细胞的体外受精。序贯共培养体系和输卵管上皮细胞培养组2-细胞率都显著高于单独培养液组和子宫内膜细胞培养组(P<0.05),二者均可克服2-细胞阻滞。序贯共培养体系胚胎的囊胚体外发育率显著高于其他共培养组(P<0.05)。表明序贯共培养体系比单一体细胞共培养体系能更好地模拟体内环境,提高早期胚胎的体外发育率。

The oviduct epithelial cells and endometrial cells were isolated and cultured in vitro and constructed sequential co-culture system, and this system was used for co-culture with fertilized egg in vitro. The results showed that HTF had higher rate of fertilization corn was pared to T6（ P 〈 0.05）, suitable for cocyte fertilization in vitro. More 2-cell rate in the sequential co-culture system and oviduct epithelial cells culture system was than single media and endometrial cells culture system, they both can efficiently overcome embryo development retardation after 2-cell stage. More. embryos in the sequential co-culture system reached in the blastocyst stage were than that in other co-culture system（ P 〈 0.05）. This study demonstrated that the sequential co-culture system can better simulate the natural developing environment of embryos in vitro and improve the development rate and quality of early embryo.