应用聚合酶链反应方法检测宫颈癌患者的人乳头瘤病毒

Detection of Human Papillomavirus in Biopsy Specimens from Cervical Carcinoma by PCR-endonuclease Cleavage

目的探讨人乳头瘤病毒(human papillomavirus,HPV)在宫颈癌的发生中的作用。方法采用聚合酶链反应(PCR)-核酸内切酶分型检测宫颈癌活检组织中HPV-DNA,对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道人乳头瘤病毒(HPV)(HPV6、11、16、18)的检测和分型。结果在宫颈癌活检组织中HPV-16,18型39.1%,与正常妇女宫颈组织阳性率均为2.2%比较,差异均具有统计学意义。尖锐湿疣主要是HPV-Ⅰ(6,11型)感染,阳性率为69.2%,HPV-Ⅱ仅占4.2%。结论宫颈癌主要与HPV16及18型感染有关。

Objective To detect the human papillomavirus （HPV） in biopsy specimens of cervical carcinoma and genital verruca by PCR-endonuclease cleavage. Methods Biopsy samples of were obtained from 353 patients with cervical carcinoma, HPV were detected by PCR and endonuclease cleavage. Results The results indicated that HPV types 16, 18 were found in 138 （39.1%） 353 cervical carcinoma, while HPV types 6, 11 were found in 81 （69.2%） of 117 acuminate verruca. The HPV 16, 18 types found in normal cervical secretions of the control group all were 2. 2%, There was significant difference between the test group of cervical carcinoma and the control group of normal cervical secretion （P 〈 0. 001 ）. Acuminate verruca was mainly infected by HPV-Ⅰ （6,11type） , HPV 6, 11 types and HPV-Ⅱ from acuminate verruca were 36.0%, 4.2% positive rate respectively. Conclusions The results show that HPV 16, 18 types are associated with majority of the cervical carcinoma.