三叶青总氨基酸对四氯化碳致小鼠肝损伤的保护作用

Protective Effects of Total Amino Acids from Radix Tetrastigmae on Acute Hepatic Injury Induced by CCl_4

目的观察三叶青总氨基酸(TAART)对四氯化碳(CCl4)诱导的小鼠急性肝损伤的保护作用。方法选用CCl4致小鼠急性肝损伤模型,以测定小鼠血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性,肝脏系数,肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及对肝组织进行病理学检查等指标观察TAART对肝损伤的保护作用。结果TAART能明显降低CCl4致小鼠肝损伤血清ALT,AST值,降低肝脏系数及肝组织中MDA的含量,增加肝组织中SOD活性,减轻CCl4对肝脏细胞的病理损伤。结论三叶青总氨基酸对CCl4致小鼠急性肝损伤具有保护作用,其机制可能与其抗氧化作用相关。

OBJECTIVE To investigate the protective effects of total amino acids from Radix Tetrastigmae （TAART） on hepatic injury induced by CCl4. METHODS Mice were administrated with TAART ig 500, 250 mg · kg^-1 daily for 7 d respectlvely, and then acute hepatic injury model was induced by 0.1% carbon tetrachloride ip 20 mL·kg^-1. The protective effects of TAART on the model were evaluated by the determination of the activity of serum alanine and aspartate aminotransferase （AST and ALT） activity, liver index, the activity of SOD and the MDA content in liver, and the hepatic tissue pathology was observed. RESULTS Following the treatment with TAART, AST and ALT activities, liver index and the content of MDA in liver were obviously reduced; the activity of SOD in live was obviously increased. Furthermore, thepathological changes were also improved significantly. CONCLUSION TAART holds protective effects on the acute hepatic injury induced by CCl4 in mice. The mechanisms may be related with their reducing anti -oxidation.