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保罗样激酶基因沉默体外抑制胶质瘤细胞侵袭与生长 被引量：2

Invasion and Growth inhibition by plkl silencing in glioma cells in vitro
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摘要 目的观察保罗样激酶1基因(plk1)沉默对胶质瘤细胞株-H4体外生长的抑制作用,探讨plk1基因作为胶质瘤治疗靶点的可行性。方法化学合成小片断干扰RNA(siRNA)抑制plk1基因的表达,Western blot检测plk1蛋白质的表达变化,流式细胞仪检测H4细胞周期分布及凋亡程度的变化,体外侵袭实验检测H4细胞侵袭能力的变化,MTT法检测H4细胞增殖速度的变化。结果经siRNA作用48h后,plk1蛋白质水平明显降低;较多的H4细胞聚集于G2/M期附近(P<0.05);细胞凋亡明显上升(P<0.05);细胞体外侵袭能力下降(P<0.05);增殖速度明显缓于对照组(P< 0.05)。结论靶向plk1的siRNA可在体外抑制胶质瘤细胞H4的侵袭与增殖,plk1有可能成为新的潜在胶质瘤治疗靶点。 Objective To observe the effect of plkl gene silencing on the growth of glioma cell line- H4 in vitro and discuss the possibility of arranging plkl as therapeutic target for glioma. Methods The plkl expression was inhibited by chemically synthesized siRNA, the plkl protein level were detected by Western blot, the change of cell cycle distribution and apoptosis was measured by flow-cytometry, the H4 cell proliferation was measured by MTY assay, lastly, the ability of H4 cell invasion in vitro was measured by cell migration and invasion assay. Results After treatment with plkl siRNA, plkl protein level decreased obviously, more H4 cells accumulated at G2/M phase and showed a higher degree of apoptosis（ P 〈 0.05 ） , H4 cells treated by plkl siRNA showed lower invasion ability in vitro（ P 〈 0.05 ）, and proliferated slowerly than control group（ P 〈 0.05 ） , Conclusions siRNA targeting plkl could inhibit invasion and proliferation of H4 cells in vitro, plkl may become another novel and potential therapeutic target for glioma.
作者 张健 石松生 兰斌 刘楠 郑安 杨卫忠
机构地区 福建医科大学附属协和医院神经内科 福建医科大学附属协和医院神经外科 福建医科大学附属第一医院肿瘤外科
出处 《中华神经外科杂志》 CSCD 北大核心 2007年第6期473-475,共3页 Chinese Journal of Neurosurgery
关键词 脑胶质瘤 小片断干扰RNA 保罗样激酶1 有丝分裂 Glioma siRNA plkl Mitosis
分类号 R739.4 [医药卫生—肿瘤]
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参考文献12

  • 1兰斌,刘炳亚,朱正纲.PLK1在细胞周期生物学的研究进展[J].医学分子生物学杂志,2005,2(1):65-67. 被引量：15
  • 2Takahashi T, Sano B, Nagata T, et al. Polo-like kinase 1( PLK1) is overexpressed in primary colorectal cancers. Cancer Sci, 2003, 94: 148-152.
  • 3Weichert W, Denkert C, Schmidt M, et al. Polo-like kinase isoform expression is a prognostic factor in ovarian carcinoma. Br J Cancer, 2004, 90: 815-821.
  • 4Dietzmann K, Kirches E, von Bossanyi, et al. Increased human polo-like kinase-1 expression in gliomas. J Neurooncol, 2001, 53: 1-11.
  • 5Smith MR, Wilson ML, Hamanaka R, et al. Malignant transformantion of mammalian cells initiated by constitutive expression of the polo-like kinase. Biochem Biophys Res Commun, 1997, 234: 397-405.
  • 6Liu X, Erikson RL. Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of plk1 by siRNA. Proc Natl Acad Sci USA, 2002, 99: 8672-8676.
  • 7江玉泉,李冰,姜政,徐卫萍,刘福生,张庆林.组胺诱导胶质瘤原代培养细胞凋亡的实验研究[J].中华神经外科杂志,2005,21(5):265-268. 被引量：4
  • 8周晓平,卢旺盛,沈茜,刘建民,岳志健,许奕,洪波,姜秀峰.TIMP-2对人脑胶质瘤U87体外侵袭力的抑制作用[J].中华神经外科杂志,2005,21(1):48-50. 被引量：7
  • 9Chase D, Serafinas C, Ashcroft N, et al. The polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans. Genesis, 2000, 26: 26-41.
  • 10Neef R, Preisinger C, Sutcliffe J, et al. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. J Cell Biol, 2003, 162: 863-875.

二级参考文献36

  • 1[5]Golsteyn RM, Mundt KE, Fry AM, et al. Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function. J Cell Biol, 1995, 129(6):1 617-28.
  • 2[6]Taniguchi E, Toyoshima-Morimoto F, Nishida E. Nuclear translocation of plk1 mediated by its bipartite nuclear localization signal. J Biol Chem, 2002, 277(50):48 884-8.
  • 3[7]Roshak AK, Capper EA, Imburgia C, et al. The human polo-like kinase, PLK, regulates cdc2/cyclin B through phosphorylation and activation of the cdc25C phosphatase. Cell Signal, 2000, 12(6):405-11.
  • 4[8]Jackman M, Lindon C, Nigg EA, et al. Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol, 2003, 5(2):143-8.
  • 5[9]Chase D, Serafinas C, Ashcroft N, et al. The polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans. Genesis, 2000, 26(1):26-41.
  • 6[10]Liu X, Erikson RL.Polo-like kinase (Plk)1 depletion induces apoptosis in cancer cells. Proc Natl Acad Sci USA, 2003, 100(10):5 789-94.
  • 7[11]Smits VA, Klompmaker R, Arnaud L, et al. Polo-like kinase-1 is a target of the DNA damage checkpoint. Nat Cell Biol, 2000, 2(9):672-6.
  • 8[12]Liu X, Erikson RL. Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of Plk1 by siRNA. Proc Natl Acad Sci USA, 2002, 99(13): 8 672-6.
  • 9[13]Sumara I, Vorlaufer E, Stukenberg PT, et al. The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase. Mol Cell, 2002, 9(3):515-25.
  • 10[14]Neef R, Preisinger C, Sutcliffe J, et al. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. J Cell Biol, 2003, 162(5):863-75.

共引文献23

同被引文献37

  • 1刘林,张敏,邹萍,田蕾,刘芳华.PLK1基因沉默对K562/A02细胞周期、增殖和耐药性的影响[J].中国实验血液学杂志,2006,14(2):241-246. 被引量：5
  • 2沈剑虹,罗其中,包映晖,江基尧,童菊芳,杨绍峰.siRNA抑制神经元NgR mRNA的时效关系和毒性研究[J].中华神经外科杂志,2006,22(11):695-698. 被引量：8
  • 3Yim H,Erikson RL. Polo - like kinase 1 depletion induces DNAdamage in early S prior to caspase activation [ J ]. Mol Cell Biol,2009,29(10):2609 -2621.
  • 4Wu ZQ,Liu X. Role for Plkl phoaphorylation of Hbol in regulationof replication licensing [ J ]. Proc Natl Acad Sci USA, 2008,105(6):1919 -1924.
  • 5Li H, Wang Y, Liu X. Plkl -dependent phosphorylation regulatesfunctions of DNA topolsomerase Ila in cell cycle progression[ J]. JBiol Chem,2008,283(10) :6209 -6221.
  • 6Feng YB,Lin DC,Shi ZZ,et al. Overexpression of PLK1 is associ-ated with poor survival by inhibiting apoptosis via enhancement ofsurvivin level in esophageal squamous cell carcinoma [ J ]. Int JCancer,2009,124(3) :578 -588.
  • 7Shi W,Alajez NM,Bastianutto C,et al. Significance of Plkl regula-tion by miR -100 in human nasopharyngeal cancer[ J]. Int J Canc-er,2010,126(9) :2036 -2048.
  • 8Renner AG,Dos Santos C,Recher C,et al. Polo - like kinase 1 isoverexpressed in acute myeloid leukemia and its inhibition prefer-entially targets the proliferation of leukemic cells[ J]. Blood,2009,114(3):659 -662.
  • 9Schmit TL,Zhong W,Nihal M, et al. Polo - like kinase 1 ( PLK1 )in non - melanoma skin cancers [ J ]. Cell Cycle,2009,8 (17):2697 -2702.
  • 10Wang ZX,Xue D,Liu ZL,et al. Overexpression of polo - like ki-nase 1 and its clinical significance in human non - samll cell lungcancer[ J]. Int J Biochem Cell Biol,2012,44(1) :200-210.

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二级引证文献13

中华神经外科杂志
2007年 第6期

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