PCR技术用于单个卵裂球基因诊断的实验研究

Application of the single blastomere PCR for preimplantation genetic diagnosis.

目的建立单卵裂球PCR技术,为开展单基因病的着床前遗传学诊断奠定基础。方法共对103个卵裂球进行了PCR扩增;其中30个扩增KG8位点,33个扩增D16S291位点,40个扩增D16S423位点。结果单卵裂球扩增KG8、D16S291和D16S423的成功率分别为86.7%、87.9%和87.5%。结论建立的单卵裂球PCR技术是稳定可靠的,可以用于单基因病的着床前遗传学诊断。

This study is to set up a technique of single blastomere PCR, and to provide a basis for preimplantation genetic diagnosis. Methods： We amplified 103 blastomeres by PCR, including 30 blastomeres for KGS, 33 blastomeres for D16S291 and 40 blastomeres for D16S423. Results： The amplification success rate of the single blastomere for KGS, D16S291 and D16S423 were 86.7%, 87.9% and 87. 5%, respectively. Conclusion： Single blastomere PCR is stable and reliable, and it can be used for nreimnlantation genetic diagnosis.