基因重组乳链球菌防龋疫苗的研究Ⅶ.基因重组乳链球菌免疫BALB/c鼠的实验研究

Study on the vaccine against dental caries of recombinant streptococcus lactis and artificial active immunization Ⅶ. Artificial active immunization with vaccine against dental caries of generecombinant S.lactis in experimental animals

目的：观察基因重组乳链球菌的免疫原性和免疫反应性。方法：对实验动物ＢＡＬＢ／ｃ鼠进行基因重组乳链球菌防龋疫苗的灌胃免疫，并以酶联免疫吸附实验进行鼠血清和唾液中抗特异性ＰＡｃ－ＩｇＧ测定。结果：接种重组乳链球菌ＨＬ１０２、ＨＬ１０７组血清中抗ＰＡｃ－ＩｇＧ明显高于乳链球菌免疫组（Ｐ＜０．０１），与接种变形乳链球菌组产生同样的ＩｇＧ反应（Ｐ＞０．０１）。结论：基因重组乳链球菌具有变形乳链球菌表面蛋白抗原的免疫原性，能够在ＢＡＬＢ／ｃ鼠中产生特异性免疫反应和免疫表达产物抗特异性ＰＡｃ－ＩｇＧ。

Aim: To test the titers of PAc-specific IgG antibodoies and immunogenicity and immunoreactivity of recombinant S.lactis. Methods: The intragastric immunization was performed with vaccine against dental caries of generecombinant S.lacis in BALB/c mice. Saliva and serum samples were collected and assayed by enayme-linked immunosorbent assay (ELISA). Results: Combinant organisms HL102 and HL107 were more effective in inducing PAc-specific serum IgG antibodies in BALB/c mice when given intragastrically than S.lactis LM0230( P <0.01), while had the same effect as S. mutans ( P >0.01). Conclusions: The generecombinant S. lacis possesses an immunogenicity of S. mutans surface protein antigen. Moreover, the recombinant organism is able to induce specifical immune response in BALB/c mice and produce immune expression product PAc- specific IgG.