当归芍药散防治老年期痴呆的物质基础与作用机理研究Ⅸ——当归芍药散精简方多糖部位的抗氧化作用研究

Study on Material Basis and Mechanism of Action of Dangguishaoyaosan in the Prevention and Treatment of Senile Dementia Ⅸ:The Anti-oxidative Activity of The Polysaccharides of FBD

目的:研究当归芍药散精简方(FBD)多糖部位(P1)的抗氧化作用。方法:采用MTT法测定H2O2所致氧化损伤后PC12及ECV304细胞的活力;分别采用黄嘌呤氧化酶法和硫代巴比妥酸比色法测定环磷酰胺损伤后小鼠脑组织中超氧化物歧化酶(SOD)的活性和丙二醛(MDA)含量。结果:200 mg/kg、400 mg/kg FBD多糖部位10%含药血清均能对细胞的氧化损伤起到显著的保护作用;200 mg/kg、400 mg/kg FBD多糖部位能显著降低氧化损伤小鼠脑内MDA的含量,有增加小鼠脑内SOD活性的趋势。结论:FBD多糖部位具有显著的抗氧化作用,这可能是其防治血管性痴呆的作用基础。

Objective： To observe the anti-oxidative activity of the polysaccharides（P1 ） of FBD. Methods：The oxidative injured model of PC12 and ECV304 ceils induced by H2O2 were used to measure the oxidation prevention of FBD Ps on ceils which was observed with MrlT metherd. The oxidative injured model of mice induced by cyclophosphamide was used, the activities of SOD and MDA in brains were measured. Results. The FBD Ps has noteable functions of oxidation prevention on the oxidative injured model of PC12 and ECV304 cells in concentrations of 200 mg/kg and 400 mg/kg FBD t Ps. They also can rise the activity of SOD and decline the content of MDA. Conclusions ： FBD Ps has notable function for oxidation prevention. This maybe the basis of its function for VD prevention.