加味温胆汤含药脑脊液抗Aβ细胞毒性作用及其机制的实验研究

Cerebrospinal Fluid Containing Modified Wendan Decoction for Protection Against β-amyloid_(25～35)-induced Injury in NG108-15 Cells

目的：采用脑脊液药理学的方法探讨加味温胆汤治疗AD的作用机制。方法：运用体外细胞培养的方法,观察加味温胆汤含药脑脊液对Aβ25~35诱导的NG108-15细胞损伤的影响。结果：模型组p-JNK和p53的表达均增高,经治疗后均显著降低（P〈0.01）,细胞被阻滞在S期。结论：加味温胆汤含药脑脊液神经保护作用与下调p-JNK和p53的水平有关。

Objective： To explore the effective mechanism of Modified Wendan Decoction in treating AD with ＂Cerebrospinal Fluid Pharmacology＂. Methods： NG108-15 cells were cultured in vitro detecting the action of Modified Wendan Decoction for protection against Aβ25-35-induced injury in NG108-15 cells. Results： Both of p-JNK and p53 are obviously up-regulated in the model group and both are dose-dependently down-regulated after treated（ P 〈 0.01 ）. The cells are arrested in S phase. Conclusion： The JNK signal pathway involved in the cell apoptosis process induced by the Aβ25-35 fragment, Cerebrospinal fluid containing Modified Wendan Decoction can down-regulate the level of p-JNK and p53,promote cells transfering from S phase to G2 phase. It can neutrolize the cytotoxicity to some degree. Its possible mechanism maybe relevant to the activation of JNK signal pathway and down-regulation of p53.