曼陀罗总DNA导入烟草获得属间杂种TD801

An new datura-tobacco hybrid TD801 obtained using total DNA transformation

采用花粉管通道法,将毛曼陀罗(Daturainnoxia Mil1)的总DNA导入烟草栽培品种Basma,经过田间筛选,得到属间杂交品系TD801;TD801的农艺性状、常规化学成分与受体亲本Basma具有一定差异,GC-MS检测表明,TD801含有供体毛曼陀罗中特有的药用成分:阿托品和东莨菪碱。选用75个随机引物分别对TD801、毛曼陀罗、Basma进行RAPD扩增,在TD801中共扩出454条带,其中21条带只在毛曼陀罗中特异出现,但在烟草中找不到同源条带。证明部分毛曼陀罗的DNA片段在TD801基因组中进行了整合。

Hairy datura （Datura innoxia Mil1 ）, a traditional medicinal plant for curing cough and asthma through burning and fumigating was crossed to oriental tobacco Basma by total DNA transformation . A new hybrid descendent, designated as TD801, was obtained after 3 years＇ selection. TD801 inherited certain excellent characters from both tobacco and hairy datura phenotypically. Chemical analysis of TD0801 and Basma showed less noticeable changes in other chemical contents, such as sugar, protein and nicotine. Typical medicinal substances of hairy datura, e.g. atropine and scopolanine, were detected only in hybrid TD801 but not in Basma. 75 random DNA markers were used to detect the genomes of tobacco, hairy datura and hybrid TD801 with the objective to assay their genetic diversity. Total 454 bands were produced in TD801 and 21 bands （4. 625 % ） were discovered only in hairy datura but not in Basma, which proved that some DNA fragments of hairy datura were integrated into TD801 genome. This revealed a potential application of TD801 plants as special tobacco variety that may help to protect to respiration system when smoked.