免疫抑制剂对嗜铬细胞瘤12细胞和L929细胞增殖的影响

EFFECTS OF IMMUNOSUPPRESSANTS ON PROLIFERATION OF PHEOCHROMOCYTOMA 12 AND L929 CELLS

目的 初步探讨多种免疫抑制剂对嗜铬细胞瘤12（pheochromocytoma 12，PC12）细胞和L929细胞增殖的影响。方法 对数生长期PC12细胞和L929细胞传代，取细胞株复苏后第3代细胞均以1×10^6／ml密度接种于培养板中，分别加入10、10、10^-7和10^8mol／L环孢菌素A（cyclosporin A，CsA），10^-6、10^-7、10^-8和10^-9mol／LFK506以及10^-3、10^-4、10^-6和10^-8mol／L甲基强地松龙，并设立空白对照组。于培养24、48和72h后，取各浓度药物作用的细胞，采用MTT法检测细胞增殖。结果 高浓度（10mol／L）甲基强地松龙和较低浓度（10^-8～10^-7mol／L）CsA，在给药后48h内对PCI2细胞增殖有明显促进作用，此后促增殖作用不明显；而各个浓度的FK506均无促进PCI2细胞增殖的作用。高浓度甲基强地松龙（10^-3mol／L）和CsA（10^-6～10mol／L）作用24h后，对L929细胞增殖有显著抑制作用，FK506仅在较高浓度（10^-6mol／L）有一过性（仅出现于给药后48h）促进L929细胞增殖的作用。结论 10^-3mol／L甲基强地松龙和10^-8～10^-7mol／LCsA能够在短时间内促进PC12细胞增殖，而10^-3mol／L甲基强地松龙和10^-6～10^-5mol／L CsA对L929细胞增殖有显著抑制作用。

Objective To explore the effects of several immunosuppressants on the proliferation of pheochromocytoma 12 （PC12） and L929 cells. Methods Different concentrations of methylprednisolone （10 3,10 4, 10^-6and 10^-8 tool/L）, cyclosporin A（CsA, 10^-6,10^-6 10^-7 and 10^-8 mol/L） and FK506 （10^-6,10^-7 10^-8 and 10^-9 mol/L） were administrated to the PC12 and L929 cells, while control group was given no drugs. At 24, 48 and 72 hours after administration, the cell proliferation was measured with MTT methods respectively. The results were compared and analyzed statistically. Results High concentration methylprednisolone （10^-3 mol/L ） and low concentration CsA （10^-8-10^-7 mol/L ） could promote the proliferation of PC12 cells within 48 hours after administration, after that, the proliferation effects were no longer significant. There were no promotion effects for different concentrations of FK506. Under high concentrations, both CsA （10^-6-1× 10^-5 mol/L） and methylprednisolone （10^-3 mol/L） could significantly inhibit the proliferation of L929 cells after 24 hours of administration. And high concentration （10^-6 mol/L） FK506 could promote the proliferation of L929 cells transitorily （only for 48 hours after administration）. Conclusion 10^-3 mol/L methylprednisolone and 10^-8-10^-7 mol/L CsA can promote the proliferation of PC12 cells for a short period of time. Both 10^-3 mol/L methylprednisolone and 10^-6-10^-5mol/L CsA can significantly inhibit proliferation of L929.