胰岛素样生长因子Ⅱ受体在卵巢癌细胞株中的表达及全反式维甲酸对其表达的影响

Changes of insulin like growth factor Ⅱ receptor expression in ovarian cancer cell lines by all transretinonic acid.

目的：探讨胰岛素样生长因子Ⅱ受体在卵巢癌细胞中的表达以及全反式维甲酸对其的影响。方法：应用ＮｏｒｔｈｅｒｎＢｌｏｔ技术检测卵巢癌细胞株３Ａｏ，Ａｏ有无胰岛素样生长因子Ⅱ受体以及全反式维甲酸对其的影响。结果：卵巢癌Ａｏ细胞株高表达胰岛素样生长因子Ⅱ受体，３Ａｏ细胞则较低。全反式维甲酸明显引起Ａｏ细胞胰岛素样生长因子Ⅱ受体下降，而３Ａｏ细胞差异不明显。结论：卵巢癌细胞株３Ａｏ和Ａｏ均表达胰岛素样生长因子Ⅱ受体，全反式维甲酸引起Ａｏ细胞株胰岛素样生长因子受体下降，提示胰岛素样生长因子Ⅱ受体参与了全反式维甲酸诱导卵巢癌Ａｏ细胞凋亡过程。

Objective To investigate the changes of insulin like growth factor Ⅱ receptor expression in ovarian cancer cell lines by all transretinonic acid. Methods The expression of insulin like growth factor Ⅱ receptors was tested by using the northern blot analysis. Results The expressions of insulin like growth factor Ⅱ receptors were detected by northern blot analysis in both 3AO and AO ovarian cancer cells.After exposed to all transretinonic acid for 48 hours,the levels of insulin like growth factor Ⅱ receptors were remarkedly decreased in AO cells.No significant change in expression of insulin like growth factor Ⅱ receptor was detected in 3AO cells. Conclusions All transretinonic acid could diminish the expression of insulin like growth factor Ⅱ receptors in AO cells.This may indicate involvement of insulin like growth factor Ⅱ receptor in all transretinonic acid induced apoptosis in AO cells.