日龄对仔猪股骨骨髓组织抗菌肽PR-39 mRNA表达的影响

Effect of day age on PR-39 gene expression in femur marrow of piglets

为探明日龄对仔猪非特异性免疫形成的影响,分别于仔猪出生当天,3,14,23,28 d,随机屠宰杜长大仔公猪4头,采集股骨骨髓组织.用一优化的RT-PCR方法,以18S rRNA作内标,半定量分析不同日龄仔猪骨髓组织抗菌肽PR-39 mRNA表达的差异.结果表明:不同日龄间仔猪骨髓组织PR-39 mRNA相对表达量存在明显的差异.出生时最低,吮吸初乳2 d后显著增加,14日龄时较3日龄显著下降,随后随日龄的增加,其表达量呈上升趋势.

In the present investigation an optimized semi-quantitative RT-PCR was applied to detect the PR-39 gene expression difference in femur marrow of piglets at different ages by using 18S rRNA as inner marker. Samples were taken at birth, 3, 14, 23, 28 days of age respectively. The results showed that day age affected PR-39 mRNA levels in femur marrow significantly （P 〈 0.05）, the expression of PR-39 mRNA was the lowest at birth, then increased remarkably at 3 days of age, but the expression at 14 d-old was lower than that of 3 days （P 〈 0.05）, and increased thereafter.