聚己内酯电纺纤维支架材料对骨髓基质细胞增殖及分化的影响

Effect of polycaprolactone-based electrospun nanofibrous framework on proliferation and differentiation of bone marrow stromal cells in vitro

目的:观察聚己内酯(PCL)电纺纤维支架材料对骨髓基质细胞(BMSCs)增殖及分化特点的影响,评价其作为骨组织工程的支架材料的应用前景.方法:将兔BMSCs与PCL电纺纤维支架材料在培养板内共培养.采用形态学观察、MTT法及ALP检测等方法检测BMSCs在PCL电纺纤维表面的粘附、增殖和分化能力.结果:体外培养的BMSCs能在PCL电纺纤维表面正常粘附和增殖,并且表达较高的碱性磷酸酶活性.结论:PCL电纺纤维适合作为支架材料应用于BMSCs为种子细胞的组织构建.

AIM： To evaluate the effect of polycaprolactone （PCL）-based electrospun nanofibers on proliferation and differentiation of bone marrow stromal cells （ BMSCs ）. METHODS： BMSCs were cultured in vitro and implanted on PCL-based electrospun nanofibers. The proliferation and differentiation of BMSCs were detected through scanning electron microscopy （SEM）, MTT assay and the detection of alkaline phosphatase （ALP） activity. RESULTS： BMSCs could adhere to and proliferate on the surface of PCL-based electrospun nanofibers. BMSCs had a higher ALP activity. CONCLUSION： PCL-based electrospun nanofiber is a suitable framework material for BMSCs to fabricate tissue engineered bone.