摘要
采用PCR-RFLP法对苏太猪SLA-DQB基因外显子2的PCR产物进行分析,结果显示,SLA-DQB基因外显子2经RsaⅠ酶切后,共分出4种基因型;经HaeⅢ酶切,共分出3种基因型。χ2适合性检验结果表明,苏太猪SLA-DQB基因外显子2在RsaⅠ和HaeⅢ酶切位点都已经达到了Hardy-Weinberg平衡状态。在RsaⅠ和HaeⅢ酶切后分出的4种组合带型中,组合带型为BBEE的种母猪第3胎总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重均显著高于组合带型为AADD的个体(P<0.05),也高于组合带型为ABDE和ACDE的个体,但是差异不显著(P>0.05)。
The exon 2 of SLA-DQB gene in Sutai pigs was analyzed by PCR-RFLP method, the results showed that the exon 2 of SLA-DQB gene digested by restriction endonuclease Rsa Ⅰ and Hae Ⅲ could be divided into four kinds of genotypes and three kinds of genotypes in Sutai pigs respectively. The results of χ^2 indicated that the frequencies of patterns digested by Rsa Ⅰ and Hae Ⅲ fit with Hardy-Weinberg equilibrium in Sutai population. Four kinds of combined patterns were observed in Sutai pigs. The correlation between these combined patterns and the reproductive performances of Sutai pigs was analyzed, the results showed that reproductive performances （the total litter, number of viable pigs, litter weight at birth, number of ablactation pigs and litter weight after ablactation in the third litter） of Sutai pigs with BBEE combined pattern were significant higher than those with AADD combined pattern, also higher than the pigs with ABDE combined pattern and ACDE combined pattern, but were no significant difference in reproductive performances between pigs with AADD combined pattern and the pigs with ABDE and ACDE combined patterns.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第7期636-640,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省高技术研究(农业)(BG2006302)
