水稻品种“佳辐占”应答细菌性条斑病病原菌侵染的蛋白质组学分析

Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。

Rice bacterial leaf streak（BLS） caused by the pathogen Xanthomonas oryzae pv. oryzicola （Xooc） is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan （ Oryzae sativa L. ） highly resistant to the disease, were infected by ＂89773-1-1＂ strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two-dimensional electrophoresis. It was found that there were thirty-eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was ＂new＂. Of the thirty-eight responsive proteins, thirty-three were identified by MALDI-TOF-MS and database searching. Based on the predicted function, we grouped them into four dusters： signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological reactions, including signal recognition and transduction, antioxidant reaction, carbonhydrate metabolism, cell-wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduction and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis-related（PR） proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistant-related genes in rice to BLS.