摘要
目的:检测caveolin-1基因mRNA在小鼠牙发育不同时期间充质细胞中的表达,初步探讨caveolin-1在小鼠牙胚发育过程中的作用。方法:以E9.5第一鳃弓间充质细胞及E16.5下颌第一磨牙牙胚细胞作为研究发育机制的模型,应用RT-PCR技术检测caveolin-1 mRNA的表达。结果:caveolin-1 mRNA在两种细胞中均有表达,其在牙胚细胞中的表达水平高于第一鳃弓间充质细胞。结论:caveolin-1可能在牙发育过程中发挥作用。
Objective： To study the expressidon of caveolin-1 mRNA in tooth development of mouse with different mesenchyrnal cells. Methods： Total RNA was extracted from E9.5 ectoderm mesenchyrnal cells and E16.5 tooth germ mesenchyrnal cells of mouse and the gene mRNA was determined by using RT-PCR technique. Results： caveolin-1 mRNA was detected in both mesenchyrnal cells and its level in tooth germ mesenchymal cells is higer than that in ectoderm mesenchyrnal cells. Conclusion： caveolin-1 may play a important role in tooth development of mouse.
出处
《现代生物医学进展》
CAS
2007年第7期1028-1030,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金(30572051)
