Survivin基因在系统性红斑狼疮患者中的异常表达

The Abnormal Expression of Survivin Gene in SLE Patients

目的:检测Survivin基因在系统性红斑狼疮(SLE)患者中的表达变化,探讨Survivin基因在SLE发病过程中的作用机制。方法:抽取32例正常健康对照人群和26例SLE患者的外周血,分离单个核细胞,提取总RNA。半定量RT-PCR法检测Survivin基因的表达,以GAPDH基因为内对照,结果以Survivin基因与GAPDH基因的RT-PCR产物的灰度比值表示。同时分析Survivin基因与SLE患者病情活动度的三个实验室指标dsDNA、补体C3和C4的相关性。结果:半定量RT-PCR显示Survivin基因mR- NA表达在SLE患者组为0.83±0.61,正常对照组为0.49±0.59,SLE患者的Survivin基因mRNA的表达明显高于正常对照组(P<0.05)。SLE患者的抗dsDNA抗体水平与Survivin基因表达呈正相关(R=0.62,P<0.01),C3水平则与Survivin基因表达呈负相关(R=-0.41,P<0.05),而C4水平则与Survivin基因表达无显著相关(R=-0.28,P>0.05)。结论:Survivin基因在SLE患者外周血中异常高表达,可能在SLE的发病机制中起着一定的作用,且其高表达与SLE患者的病情活动度有关。

Objective： To study the expression of survivin gene in SLE patients and to explore the role of survivin gene in SLE pathogenesis. Methods： Total RNAs were extracted from peripheral blood mononuclear cells of 32 healthy controls and 26 SLE patients. Survivin gene expression was detected by semi-quantitative RT-PCR analysis while GAPDH house-guarded gene expression was served as internal control. The relationship between the survivin gene expression and the levels of anti-dsDNA antibody, C3 or C4 was also analyzed. Results： Semi-quantitative RT-PCR results revealed a significant increase in the sttrvivird GAPDH ratio of up to 1.7 times in the SLE group compared with the healthy control （0.83±0.61 vs 0.49±0.59 P〈0.05）. The expression of survivin gene was positively related with the level of anti-dsDNA antibody （R=0.62,P〈0.01） and negatively to the level of C3 （R=-0.41,P〈0.05）, while no relationship was showed between sttrvivin gene expression and C4. Conclusions： The expression of survivin gene is up-regulated in SLE patients, which may play a role in SLE pathogenesis and be associated with the disease activity of these SLE patients.