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“睡美人”转座子的研究进展 被引量：6

Advances in studies of Sleeping Beauty transposon
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摘要 “睡美人(Sleeping Beauty,SB)”转座系统是Tc1/mariner转座子超家族中的一员,已经失活了一千多万年。1997年,Ivics等根据积累的系统发生数据,利用生物信息学的方法,对其进行分子重建,终于唤醒了其转座活性。近年来对“睡美人”转座系统的转座效率和转座机理进行的研究,已证明SB转座子在基因筛选,转基因及基因治疗等领域具有广阔的应用前景。文章重点论述了SB转座子在结构及其优化、转座机制和应用等方面的进展,同时对其研究中出现的各种问题进行了总结并提出了一些解决方案。 The Sleeping Beauty （SB） transposon is a Tcl/mariner family transposon that has been transpositionally inactive for over 10 million years. The SB transposon system was awakened from inactiveTcl-like transposable elements by using molecular phylogenetic data in 1997. Recent studies on its transposition efficiency and mechanism have shown its broad applications in vertebrate animals for gene-screening, gene transfer, and human gene therapy. In this review, we summarize our current knowledge of Sleeping Beauty, such as structure, transposition mechanism and potential applications, and bring forward some means aiming at the limitations of transposon technology.
作者 谢飞 高波 宋成义 陈国宏
机构地区 扬州大学动物科学与技术学院
出处 《遗传》 CAS CSCD 北大核心 2007年第7期785-792,共8页 Hereditas（Beijing)
关键词 “睡美人”转座子 脊椎动物 转座机制 Sleeping Beauty transposon vertebrates transposition mechanism
分类号 Q75 [生物学—分子生物学]
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参考文献44

  • 1McClintock B.Chromosome organization and gene expression.Cold Spring Harbor Symp Quant Biol,1951,16(3):13-17.
  • 2Yant SR,Meuse L,Chiu W,Ivics Z,Izsvak Z,Kay MA.Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system.Nat Genet,2000,25(1):35-41.
  • 3Fischer SE,Wienholds E,Plasterk RH.Regulated transposition of a fish transposon in the mouse germ line.Proc Natl Acad Sci USA,2001,98(12):6759-6764.
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  • 5Ivics Z,Hackett PB,Plasterk RH,Izsvak Z.Molecular reconstruction of Sleeping Beauty,a Tcl-like transposon from fish,and its transposition in human cells.Cell,1997,91(4):501-510.
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二级参考文献16

  • 1朱乾浩.转座子在植物基因分离中的应用研究进展[J].生物工程进展,1996,16(2):22-25. 被引量：6
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2007年 第7期

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