人红白血病细胞来源的DC对细胞因子诱导的杀伤细胞逆转多药耐药的影响

Effect of K562/ADR derived from human dendritic cell on CIK cells reversing multidrug resistance

目的:探讨人红白血病细胞(K562/ADR)来源的DC与CIK细胞体外共培养后对多药耐药逆转影响。方法:K562敏感株和K562/ADR耐药细胞株,在含细胞因子GM-CSF(1000u/mL)、IL-4(500u/mL)和TNF-α(100ng/mL)的RPMI1640完全培养液中诱导分化成DC。同时将PBMC在细胞因子诱导下培养成细胞因子诱导的杀伤细胞(CIK),与成熟DC进行共培养。采用MTT法测定免疫效应细胞对靶细胞杀伤率、耐药性逆转。流式细胞术检测靶细胞的Pgp表达及细胞内的ADR浓度,RT-PCR检测MDR1基因表达状况。结果:K562/ADR来源的DC与CIK细胞共培养后,细胞增殖速度明显加快,17d以后两者的增殖倍数呈现明显差异。效应细胞对靶细胞的生长抑制率高达78.9%,与单纯CIK细胞相比较显示出高特异免疫杀伤作用。结论:CIK+K562/ADR-DC对P-gp高表达K562/ADR耐药细胞株具有特异性的细胞毒作用,有效提高了细胞内的ADR含量,下调了P-gp、mdr-1表达,从而使免疫效应细胞体外逆转多药耐药的效果得以显现。

Objective To explore the reversion effect of human dendritic cell derived from human erythroleukemia cell （K562/ADR） co-cultured with CIK cells on multidrug resistance. Methods Resistant K562/ADR cell and sensitive K562 cell were induced from differentiating to DC in complete RPMI 1640 culture medium supplemented with GM-CSF（1000 u/mL）, IL-1 （500 u/mL） and TNF-α （100 ng/mL）. At the same time, with the induction of cytokines, PBMCs were induced into CIK, and then co-cultured with mature DC. Bill rate of immune effector cells on target cells was measured with MTT. The expression of Pgp and intracellular concentration of ADR in target cells were detected with flow cytometry. And the gene expressions of MDR1 were detected with RT-PCR. Results When tumor antigen of K562/ADR cell line loaded on DC cocultured with CIK, the cell proliferation obviously accelerated, and the multiplications showed obvious differences after 17 days. The growth inhibition ratio of effector cells on target cells reached 78.9%, and showed higher and more specific growth inhibiting effect compared to simple CIK. Conlusion CIK q-K562/ADR-DC has a specific cytotoxicity on K562/ADR drug-resistant cell lines with high P-gp expression,notably increases the content of ADR while the expression of P-gp and mdr-r are declined. The vitro MDR reversal of immune cells is shown significantly.