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小麦种子中几丁质酶基因的扩增及序列分析 被引量：1

Gene cloning and sequence analysis of chitinase gene in wheat seed
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摘要 根据http://www.tigr.org中与小麦几丁质酶基因相关的序列TC187877,设计引物,分别从小麦品种Gamenya和苏麦3号中扩增到大小约为1 000bp的片段。经序列测定和软件分析比较,发现这些片段所编码的蛋白质氨基酸序列,都有CHITINASE-19.1和CHITINASE-19.2的基序,为第II类几丁质酶基因。扩增的核酸序列在GenBank上发表,登录号分别为AY973229和AY973230。 Based on chitinase gene-relative TC187877 from http：//www.tigr.org,two pairs of primers were designed,and two 1000bp fragments were amplified from seed genomic DNA of Gamenya and Sumai 3 by PCR method.The two amplified fragments belong to Chitinase II because of their CHITINASE-19.1and CHITINASE-19.2 motifs at the amino acid level through sequencing and sequence analysis.The amplified sequences were deposited in GenBank（GenBank accession number AY973229 and AY973230 respectively）.
作者 易图永 池田逹哉
机构地区 湖南农业大学生物安全科技学院 日本西部农业研究中心育种工学研究室
出处 《生物信息学》 2007年第1期1-4,共4页 Chinese Journal of Bioinformatics
基金 湖南省教育厅日元贷款项目资助(20040121)
关键词 小麦种子 几丁酶基因 扩增 序列分析 Wheat seed chitinase gene amplify sequence analysis
分类号 S512.1 [农业科学—作物学] Q754 [生物学—分子生物学]
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共引文献89

同被引文献21

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生物信息学
2007年 第1期

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