降解烟碱的YC-68菌株16 S rDNA序列分析及鉴定

The 16 S rDNA Sequence Analysis and Identification of the Nicotine Degrading Bacterial Strain YC-68

从烟叶调制过程中温度达68℃阶段的叶片上分离得到一株能降解烟碱的菌株,编号为YC-68,该菌经常规的形态、生理生化分析,确定为芽孢杆菌。根据该菌16 S rDNA恒定区的保守性设计了一对通用引物,扩增其16 S rDNA序列,将扩增的产物进行回收后进行测序,把测序后的结果提交到GenBank利用BLAST进行序列同源性分析。经过分析鉴定YC-68菌株为芽孢杆菌属的蜡状芽孢杆菌。

When the temperature was up to 68 ℃ during the process of modulating tobacco, the bacteria designated YC-68 was separated from tobacco, which could decompose the nicotine. Through conventional morphologic, physiological, biochemical analysis, the bacteria are defined as a Bacillus strain. According to the conservative character of its invariable area, we designed a couple of primers, which would amplify 16 S rDNA sequence. We collected the PCR product and conducted sequence analysis. Afterwards, the sequence result was submitted to GenBank to take a serial homologous analysis through BLAST. According to the analysis data, it was identified as Bacillus cereus.