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光化学疗法对内皮细胞明胶酶表达的影响 被引量：1

Effect of photochemotherapy on expression of gelatases
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摘要 目的观测光化学疗法对内皮细胞明胶酶表达的影响。方法应用RT-PCR法及明胶酶法分别观测光化学疗法对内皮细胞明胶酶mRNA及活性的影响。结果2.0-5.0 J/cm^2的长波紫外线联合100 ng/mL的8-甲氧补骨脂素可在体外明显抑制明胶酶的表达及活性。结论光化学疗法可抑制内皮细胞明胶酶的表达及活性，此抑制效应与其抗血管新生效应密切相关。 Objective To determine the effect of photochemotherapy on the expression of gelatinases. Methods Reverse transcription-polymerase chain reaction and gelatine zymography were employed to detect the effect of photochemotherapy on the expression and bioactivity of gelatinases at mRNA and protein levels respectively. Results Combination of ultraviolet A （ UVA, 0.8 - 2.0 J/cm^2 ） and 8-methoxypsoralen（8-MOP, 100 ng/mL） resulted in a decrease in the expression and bioactivity of gelatinases. Conclusion Photochemotherapy can inhibit the angiogenesis of endothelial cells through downregulating the expression and bioactivity of gelatinases.
作者 邓辉 袁定芬 闫春林 廖康煌
机构地区 上海交通大学第六人民医院皮肤科 复旦大学华山医院皮肤科
出处 《上海交通大学学报（医学版）》 CAS CSCD 北大核心 2007年第1期79-81,共3页 Journal of Shanghai Jiao tong University:Medical Science
基金 上海市教委基金(01QN03)~~
关键词 光化学疗法 血管新生 明胶酶 photochemotherapy angiogenesis gelatinases
分类号 R454 [医药卫生—治疗学]
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参考文献8

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同被引文献9

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引证文献1

上海交通大学学报（医学版）
2007年 第1期

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